Related Experiment Video
Updated: Apr 4, 2026
07:02
In Vivo Inhibition of MicroRNA to Decrease Tumor Growth in Mice
Published on: August 23, 2019
7.9K
BRAF-Activated Long Noncoding RNA Modulates Papillary Thyroid Carcinoma Cell Proliferation through Regulating Thyroid
Haitao Zheng1, Meng Wang1, Lixin Jiang1
1Department of Surgery, Yantai Yuhuangding Hospital, Affiliated with Medical College of Qingdao University, Yantai, China.
Cancer Research and Treatment
|September 2, 2015
Summary
BRAF-activated long noncoding RNA (BANCR) is upregulated in thyroid cancer and promotes tumor growth by regulating cyclin D1 and TSHR. Silencing BANCR inhibits cell proliferation and induces cell cycle arrest.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- Long noncoding RNAs (lncRNAs) play a role in tumorigenesis.
- The involvement of lncRNAs in thyroid cancer development is not well understood.
Purpose of the Study:
- Investigate the role of specific lncRNAs in papillary thyroid carcinoma (PTC).
- Examine the expression patterns of BANCR, PTCSC3, and NAMA in PTC.
Main Methods:
- Quantitative reverse transcription polymerase chain reaction (qRT-PCR) was used to measure lncRNA expression.
- BANCR-knockdown experiments were performed in a PTC cell line (IHH-4).
- Effects on cell growth, cell cycle, and gene expression (TSHR, cyclin D1) were analyzed.
Main Results:
- BANCR expression was significantly upregulated in PTC tissues compared to normal tissues.
- PTCSC3 and NAMA were significantly downregulated in PTC.
- BANCR knockdown suppressed cell growth, induced G0/G1 cell cycle arrest, and downregulated cyclin D1 and TSHR.
- BANCR interacts with EZH2, influencing TSHR expression via chromatin modification.
Conclusions:
- BANCR is implicated in PTC tumorigenesis.
- BANCR may promote PTC development by regulating cyclin D1 and TSHR.
- Targeting BANCR could be a potential therapeutic strategy for PTC.
Related Concept Videos
02:39lncRNA - Long Non-coding RNAs
10.2K
In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
10.2K
02:39lncRNA - Long Non-coding RNAs
3.9K
3.9K
01:20Synthesis and Regulation of Thyroid Hormones
9.0K
Low blood levels of the thyroid hormones — triiodothyronine (T3) and thyroxine (T4) — signal the hypothalamus to release the thyrotropin-releasing hormone (TRH). TRH then reaches the pituitary gland and stimulates the release of thyroid-stimulating hormone(TSH) into the bloodstream.
Upon reaching the thyroid gland, TSH stimulates the follicular cells' active uptake of iodide ions from the blood. The ions diffuse to the apical surface of the cells and are oxidized to iodine. The...
Upon reaching the thyroid gland, TSH stimulates the follicular cells' active uptake of iodide ions from the blood. The ions diffuse to the apical surface of the cells and are oxidized to iodine. The...
9.0K
01:18Functions of Thyroid Hormones
6.7K
The thyroid hormone (TH) plays a pivotal role in the intricate orchestration of physiological processes, exerting profound effects on development, metabolism, and homeostasis throughout different life stages.
TH is indispensable for the normal development and maturation of the skeletal, muscular, and nervous systems during fetal and childhood growth. It facilitates bone mineral turnover and regulates protein synthesis in developing tissues, contributing significantly to overall growth and...
TH is indispensable for the normal development and maturation of the skeletal, muscular, and nervous systems during fetal and childhood growth. It facilitates bone mineral turnover and regulates protein synthesis in developing tissues, contributing significantly to overall growth and...
6.7K
03:03mTOR Signaling and Cancer Progression
5.1K
The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
The mTOR pathway or the...
5.1K
01:22PI3K/mTOR/AKT Signaling Pathway
6.4K
The mammalian target of rapamycin (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1 (mTORC1) and mTOR complex 2 (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast, mTORC2 consists of a...
6.4K