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Updated: Apr 4, 2026

Anaerobic Protein Purification and Kinetic Analysis via Oxygen Electrode for Studying DesB Dioxygenase Activity and Inhibition
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Structural Basis for Oxygen Activation at a Heterodinuclear Manganese/Iron Cofactor

Julia J Griese1, Ramona Kositzki2, Peer Schrapers2

  • 1From the Stockholm Center for Biomembrane Research, Department of Biochemistry and Biophysics, Stockholm University, SE-106 91 Stockholm, Sweden.

The Journal of Biological Chemistry
|September 2, 2015
PubMed
Summary

R2-like ligand-binding oxidases (R2lox) with manganese/iron cofactors self-assemble and activate oxygen. Structural analysis reveals cofactor flexibility and potential in vivo metal utilization based on availability.

Keywords:
X-ray absorption spectroscopyX-ray crystallographyferritinmetalloproteinsmall angle x-ray scattering (SAXS)

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