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Updated: Apr 4, 2026
Optimized Incorporation of Alkynyl Fatty Acid Analogs for the Detection of Fatty Acylated Proteins using Click Chemistry
Published on: April 9, 2021
Convenient analysis of protein modification by chemical blotting with fluorogenic "click" reagents
Jun Ohata1, Farrukh Vohidov, Zachary T Ball
1Department of Chemistry, Rice University, MS 60, 6100 Main Street Houston, Texas 77005, USA. zb1@rice.edu.
Abstract:
Direct visualization of bioorthogonal alkyne or azide handles using fluorogenic azide-alkyne cycloaddition conducted on the surface of a blot membrane. The method eliminates the need for separation steps to remove excess small molecule reagents before attachment of antigen molecules or other visualization handles, and is especially useful for the analysis of peptides and small proteins. A variety of potential fluorogenic reagents are assessed, and sensitivity (<0.1 picomole) similar to current commercially available fluorescence imaging methods is possible.
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