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Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction
Published on: October 6, 2022
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Detection of thrombin based on aptamer isothermal exponential signal amplification technique
Xiaotong Shen1, Menghua Zhang, Shuyan Niu
1Shandong Provincial Key Laboratory of Biochemical Analysis, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, P. R. China. chemsxt1990@163.com.
The Analyst
|September 2, 2015
Abstract:
In this report, a strategy based on an aptameric molecule switch to realize homogeneous and isothermal signal exponential amplification and protein detection has been described. This method was simple and sensitive, allowing detection of as low as 2.6 × 10(-12) M thrombin.

