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Method with high-throughput screening potential for antioxidative substances using Escherichia coli biosensor

Jenni Tienaho1, Tytti Sarjala2, Robert Franzén3

  • 1Department of Chemistry and Bioengineering, Tampere University of Technology, Korkeakoulunkatu 8, FI-33101 Tampere, Finland; Natural Resources Institute Finland (Luke), Parkano Research Unit, Kaironiementie 15, FI-39700 Parkano, Finland.

Journal of Microbiological Methods
|September 2, 2015
PubMed
Summary

A novel biosensor method enables rapid, real-time screening of antioxidant compounds. This high-throughput technique using Escherichia coli offers reliable results for discovering bioactive substances.

Keywords:
Antioxidative activityBacterial biosensorBioscreeningMicroplate technique

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Area of Science:

  • Microbiology
  • Biotechnology
  • Biochemistry

Background:

  • Antioxidative compounds are crucial for health and disease prevention.
  • Screening for these compounds often involves time-consuming methods.
  • Developing rapid and reliable screening techniques is essential.

Purpose of the Study:

  • To describe a new method for rapid, real-time screening of antioxidative properties.
  • To utilize a recombinant Escherichia coli DPD2511 biosensor for this purpose.
  • To establish a high-throughput screening technique for bioactive compounds.

Main Methods:

  • Development of a recombinant Escherichia coli DPD2511 biosensor.
  • Implementation of a microplate-based screening technique.
  • Focus on real-time analysis without lengthy pre-incubations.

Main Results:

  • The biosensor enables rapid and real-time assessment of antioxidative activity.
  • The microplate technique streamlines the screening process.
  • The method demonstrated potential for high-throughput screening.
  • Emphasis was placed on achieving reliable and repeatable results.

Conclusions:

  • The described method offers an efficient approach for screening antioxidative properties.
  • This technique facilitates high-throughput discovery of bioactive compounds.
  • The biosensor system provides reliable and repeatable results for antioxidant screening.