Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...

Confocal Fluorescence Microscopy

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Enhancer RNA Transcription Near Segmentation Gene Enhancers Can Be Analyzed In Situ Using FISH.

G3 (Bethesda, Md.)·2026

Same author

TIM3 signaling in effector T cells acts as an immunometabolic switch in the purine degradation pathway to suppress intestinal inflammation.

Gastroenterology·2026

Same author

Johns Hopkins Center for Humanitarian Health-Lancet Commission on health, conflict, and forced displacement: health in a world of crises and impunity.

Lancet (London, England)·2026

Same author

A ZEB1-Neon knock-in uncovers traceable dynamics of epithelial-mesenchymal transition in tumors in vivo.

BMC biology·2026

Same author

Early Gut-Vascular Barrier Breakdown Precedes Colitis Onset in Murine Models.

Cellular and molecular gastroenterology and hepatology·2026

Same author

Fibroblast dynamics in colorectal cancer: stability, plasticity, and novel markers.

Oncogene·2026

Same journal

Demonstration of a quantum C-NOT gate in a time-multiplexed fully reconfigurable photonic processor.

Nature communications·2026

Same journal

Nonlinear quantum light source with van der Waals ferroelectric NbOX<sub>2</sub> (X = Br, I).

Nature communications·2026

Same journal

Antagonistic histone H2A variants and autonomous heterochromatin formation shape epigenomic patterns in Arabidopsis.

Nature communications·2026

Same journal

The long tail of nitrate pollution in groundwater challenges governance of global water quality.

Nature communications·2026

Same journal

Select microbial metabolites promote tau aggregation in a murine tauopathy model.

Nature communications·2026

Same journal

Warming climate has lengthened global intense tropical cyclone seasons.

Nature communications·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Apr 4, 2026

Light-sheet Fluorescence Microscopy for the Study of the Murine Heart

Light-sheet Fluorescence Microscopy for the Study of the Murine Heart

Published on: September 15, 2018

Hyperspectral light sheet microscopy.

Wiebke Jahr^1,2, Benjamin Schmid¹, Christopher Schmied¹

¹Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauer Strasse 108, 01307 Dresden, Germany.

Nature Communications

|September 3, 2015

Summary

This summary is machine-generated.

This study introduces a new light sheet microscopy platform for efficiently imaging multiple fluorescent markers in living organisms. The system improves image quality and fluorophore discrimination by capturing the full spectrum per pixel, outperforming traditional filter-based methods.

More Related Videos

Excitation-Scanning Hyperspectral Imaging Microscopy to Efficiently Discriminate Fluorescence Signals

Excitation-Scanning Hyperspectral Imaging Microscopy to Efficiently Discriminate Fluorescence Signals

Published on: August 22, 2019

Author Spotlight: Advancing Knowledge in Far-From-Equilibrium Materials Through Light-Sheet Microscopy

Author Spotlight: Advancing Knowledge in Far-From-Equilibrium Materials Through Light-Sheet Microscopy

Published on: January 26, 2024

Related Experiment Videos

Last Updated: Apr 4, 2026

Light-sheet Fluorescence Microscopy for the Study of the Murine Heart

Light-sheet Fluorescence Microscopy for the Study of the Murine Heart

Published on: September 15, 2018

Excitation-Scanning Hyperspectral Imaging Microscopy to Efficiently Discriminate Fluorescence Signals

Excitation-Scanning Hyperspectral Imaging Microscopy to Efficiently Discriminate Fluorescence Signals

Published on: August 22, 2019

Author Spotlight: Advancing Knowledge in Far-From-Equilibrium Materials Through Light-Sheet Microscopy

Author Spotlight: Advancing Knowledge in Far-From-Equilibrium Materials Through Light-Sheet Microscopy

Published on: January 26, 2024

Area of Science:

Biomedical Imaging
Microscopy Techniques
Developmental Biology

Background:

Studying cell and tissue development requires efficient multi-fluorophore imaging in vivo.
Sequential color acquisition with filters is inefficient and can limit imaging capabilities.

Purpose of the Study:

To develop a novel platform for efficient, high-quality imaging of multiple fluorescent markers in living organisms.
To overcome limitations of filter-based microscopy for multicolor imaging.

Main Methods:

Developed a line-scanning light sheet microscopy platform.
Acquired the entire spectrum for each pixel in a 3D volume.
Evaluated spectral sampling and determined optimal channel width (~5 nm).
Utilized spectral unmixing for data analysis.

Main Results:

The new setup significantly outperforms filter-based approaches in image quality.
Achieved superior discrimination of fluorophores.
Resolved overlapping fluorophores with nanometer resolution.
Successfully removed autofluorescence in zebrafish and fruit fly embryos.

Conclusions:

The developed spectral light sheet microscopy platform enables efficient and high-resolution multicolor imaging.
This technique enhances the study of dynamic biological processes in vivo.
It offers a powerful alternative to conventional filter-based imaging methods.