[In vitro transcription of esterase S gene in Drosophila virilis]

In vitro transcription of D. virilis esterase S gene and it's dependence on reaction conditions and template configuration was studied. Localization of major in vivo transcription initiation points were proved. In vitro transcription can start from alternative points, localised at positions -125 and -342. Dependence of the transcription efficiency of the presence of the 5'-flanking region is shown.