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Profiling Ubiquitin and Ubiquitin-like Dependent Post-translational Modifications and Identification of Significant Alterations
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Reference proteome of highly purified human Th1 cells reveals strong effects on metabolism and protein ubiquitination

Massimiliano Pagani1, Maxie Rockstroh2, Maj Schuster2

  • 1Integrative Biology Program, INGM - National Institute for Molecular Genetics, Milan, Italy.

Proteomics
|September 3, 2015
PubMed

The differentiation of human CD4(+) T cells into T helper cell subtypes and regulatory T cells is crucial to the immune response. Among subtypes, Th1 cells are dominant, representing approximately 50% of all lymphocytes. Thus far, most global proteomic studies have used only partially purified T helper cell subpopulations and/or have employed artificial protocols for inducing specific T helper cell subtypes and/or used gel-based approaches. These studies have shed light on molecular details of certain aspects of the proteome; nevertheless a global analysis of high purity primary naïve and Th1 cells by LC-MS/MS is required to provide a reference dataset for proteome-based T cell subtype characterization. The utilization of highly purified Th1 cells for a global proteome assessment and the bioinformatic comparison to naïve cells reveals changes in cell metabolism and the ubiquitination pathway upon T cell differentiation. All MS data have been deposited in the ProteomeXchange with identifier PXD001066 (http://proteomecentral.proteomexchange.org/dataset/PXD001066).

Keywords:
Cell biologyHuman T-lymphocytesImmunologyLC-MS/MSProteome reference mapTh1-cells

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