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Updated: Apr 4, 2026

A Rapid and Chemical-free Hemoglobin Assay with Photothermal Angular Light Scattering
Published on: December 7, 2016
Determination of hematocrit by voltage-induced hemolysis on a disposable electrochemical sensing strip
Chih-Wei Weng1, Bo-Chuan Hsieh, Yung-Te Hou
1Biosensor Lab, Department of Bio-Industrial Mechatronics Engineering, National Taiwan University, No. 1, Sec. 4, Roosevelt Rd., Taipei 10617, Taiwan, Republic of China. tzongjih@ntu.edu.tw.
Abstract:
Besides being a crucial parameter for surgery and clinical diagnosis, hematocrit tends to affect the analytical results of point-of-care analytical devices. Therefore, an accurate and quick method for measuring hematocrit was developed on the basis of screen-printed carbon electrodes (SPCE). An impulse DC voltage of 3.0 V was imposed on ferricyanide-coated SPCE to induce hemolysis, and the released hemoglobin reduced the ferricyanide, generating a higher oxidation current for estimating hematocrit. Hematocrit ranging from 10 to 70% can be determined in 5 s by linear sweep voltammetry (r(2) = 0.9907) or 0.8 s by 3 V of potential step voltammetry (r(2) = 0.9833).
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