Related Experiment Video
Updated: Apr 4, 2026
09:30
Bacterial Detection & Identification Using Electrochemical Sensors
Published on: April 23, 2013
29.2K
Ratiometric biosensor array for multiplexed detection of microRNAs based on electrochemiluminescence coupled with
Xiaobin Feng1, Ning Gan1, Huairong Zhang1
1State Key Laboratory Base of Novel Functional Materials and Preparation Science, Faculty of Materials Science and Chemical Engineering, Ningbo University, Ningbo 315211, PR China.
Biosensors & Bioelectronics
|September 3, 2015
Summary
A new biosensor array enables near-simultaneous detection of microRNA-21 and microRNA-141 using electrochemiluminescence and cyclic voltammetry. This method offers high sensitivity for potential clinical laboratory use in biomarker detection.
Area of Science:
- * Electrochemistry and Nanomaterials Science
- * Biosensor Technology and Analytical Chemistry
Background:
- * MicroRNAs (miRNAs) are crucial biomarkers for various diseases.
- * Accurate and sensitive detection of specific miRNAs is essential for early diagnosis.
- * Existing detection methods often lack multiplexing capabilities or require complex procedures.
Purpose of the Study:
- * To develop a novel multiplexed ratiometric biosensor array for simultaneous detection of miRNA-21 and miRNA-141.
- * To utilize electrochemiluminescence (ECL) and cyclic voltammetry (CV) for dual-signal readout.
- * To achieve high sensitivity and specificity for miRNA detection in real samples.
Main Methods:
- * Fabrication of a screen-printed carbon electrode (SPCE) array with spatial resolution.
- * Synthesis of ECL signal tags (Ru-SiO2@PLL-Au) incorporating ruthenium-doped silica and gold nanoparticles.
- * Immobilization of ferrocene-labeled hairpin DNA (Fc-HDNA) as CV signal tags and ECL quenchers.
- * Hybridization of target miRNAs with Fc-HDNA, inducing conformational changes for signal generation.
- * Ratiometric analysis of
- signal-on
- ECL and
- signal-off
- CV signals for accurate quantification.
Main Results:
- * Achieved near-simultaneous detection of miRNA-21 and miRNA-141 with high sensitivity (down to 6.3 fM and 8.6 fM, respectively).
- * Demonstrated a
- signal-on
- ECL response and a
- signal-off
- CV response for ratiometric detection.
- * Validated the biosensor's performance in real sample analysis.
- * Showcased significant potential for clinical laboratory applications.
Conclusions:
- * The developed multiplexed ratiometric biosensor array offers a sensitive and efficient platform for simultaneous miRNA detection.
- * The combined ECL and CV approach provides a reliable dual-signal readout mechanism.
- * This technology holds promise for advancing miRNA biomarker detection in clinical settings.
