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OLMALINC alleviates dexamethasone-induced osteoporosis via targeting miR-124-3p
Siyuan Ha1, Jing Xiao2, Ning Gan3
1Anesthesiology, The Wuhan Hospital of TCM Affiliated to Hubei University of Traditional Chinese Medicine, Wuhan, China.
Introduction And Objective:
This study aims to investigate the mechanism of LncRNA(lncRNAs) OLMALINC in dexamethasone (Dex)-induced osteoblast differentiation impairment and osteoporosis.
Material And Methods:
To investigate the impact of OLMALINC and miR-124-3p on Dex-treated osteoblasts, functional gain and loss experiments were conducted using MC3T3-E1 cells. Dual-luciferase reporter assays, RNA pull-down, and MS-RIP experiments were used to verify the targeting relationship between OLMALINC and miR-124-3p. RT-qPCR was conducted to analyze OLMALINC and miR-124-3p levels, as well as osteogenic regulatory factors OPG, Runx2, and ALP-related mRNA in different treatment groups. Protein expression levels were determined by Western blot analysis. Apoptosis was assessed by flow cytometry. cell viability was assessed by CCK-8.
Results:
After Dex treatment, OLMALINC levels decreased, while miR-124-3p increased. Transfection of oe-OLMALINC counteracted Dex-induced osteogenic damage by increasing cell viability, decreasing apoptosis reduction, stimulating OPG, ALP, and Runx2 stimulation. OLMALINC targeted miR-124-3p, with OLMALINC negatively regulating miR-124-3p. In turn, miR-124-3p mimic reversed the protective effect of OLMALINC against Dex-induced osteoblast dysfunction.
Conclusions:
These results indicate that the OLMALINC/miR-124-3p axis influences osteoblast differentiation in Dex-induced osteoblast differentiation impairment and osteoporosis by regulating cell viability, apoptosis, and osteogenic factors.