Chiral Recognition of L-Amino Acids on Liposomes Prepared with L-Phospholipid
Takaaki Ishigami1, Keishi Suga1, Hiroshi Umakoshi1
1Division of Chemical Engineering, Graduate School of Engineering Science, Osaka University , 1-3 Machikaneyamacho, Toyonaka, Osaka 560-8531, Japan.
Abstract:
In this study, we demonstrated that liposomes composed of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) can recognize several l-amino acids, but not their d-enantiomers, by analyzing their adsorptive behavior and using circular dichroism spectroscopy. Changes in liposomal membrane properties, determined based on fluorescent probe analysis and differential scanning calorimetry, were induced by l-amino acid binding. UV resonance Raman spectroscopy analysis suggested that the chiral recognition was mediated by electrostatic, hydrophobic, and hydrogen bond interactions, where the recognition site could therefore be constructed on the DPPC membrane. Our findings clearly indicate the potential function of liposomes in asymmetric recognition.
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