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Solid phase microextraction devices prepared on plastic support as potential single-use samplers for bioanalytical

Nathaly Reyes-Garcés1, Barbara Bojko1, Dietmar Hein2

  • 1Department of Chemistry, University of Waterloo , Waterloo, Ontario N2L 3G1, Canada.

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|September 5, 2015
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Summary

New plastic-based thin-film solid phase microextraction (SPME) devices offer cost-effective, single-use sampling for bioanalysis. These robust devices, utilizing polyacrylonitrile coatings on polybutylene terephthalate, show excellent performance in biological matrices.

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Area of Science:

  • Analytical Chemistry
  • Materials Science

Background:

  • Solid Phase Microextraction (SPME) is a vital technique in bioanalysis.
  • Development of cost-effective and biocompatible SPME materials is ongoing.
  • Existing SPME devices may have limitations in specific clinical applications.

Purpose of the Study:

  • To develop novel, single-use thin-film SPME devices on a plastic substrate.
  • To evaluate the performance and robustness of these new SPME devices for bioanalysis.
  • To explore the potential of polybutylene terephthalate (PBT) as a support material for SPME.

Main Methods:

  • Thin-film SPME devices were fabricated using polyacrylonitrile (PAN) coatings on sanded polybutylene terephthalate (PBT) supports.
  • The PBT-based SPME devices were characterized for robustness and chemical stability.
  • Quantitative analysis of doping substances in biological matrices (urine, plasma, whole blood) was performed.
  • Multiple extractions were conducted using the Concept 96 system to assess device durability.

Main Results:

  • The PBT-supported SPME devices demonstrated good robustness and chemical stability.
  • Successful quantitative analysis of doping substances in urine, plasma, and whole blood was achieved.
  • Over 20 extractions were performed on plasma and whole blood without performance decrease or coating peeling.
  • The polyacrylonitrile coating on the PBT substrate proved durable.

Conclusions:

  • New, cost-effective, thin-film SPME devices on PBT are suitable for bioanalysis.
  • These single-use samplers offer a robust and reliable alternative for clinical applications.
  • The developed SPME devices present a promising advancement in biocompatible sampling materials.