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Updated: Apr 4, 2026
09:04
Transcriptome Profiling of In-Vivo Produced Bovine Pre-implantation Embryos Using Two-color Microarray Platform
Published on: January 30, 2017
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Transcriptomic Features of Bovine Blastocysts Derived by Somatic Cell Nuclear Transfer
Byungkuk Min1, Sunwha Cho1, Jung Sun Park2
1Development and Differentiation Research Center, KRIBB, 125 Gwahak-ro, Yuseong-gu, Daejeon, 305-806, South Korea Department of Functional Genomics, University of Science and Technology (UST), 217 Gajeong-ro, Yuseong-gu, Daejeon, 305-350, South Korea.
G3 (Bethesda, Md.)
|September 6, 2015
Summary
Somatic cell nuclear transfer (SCNT) in cattle leads to incomplete reprogramming, affecting gene expression in embryos. Transcriptome analysis reveals distinct patterns in SCNT versus in vitro fertilization (IVF) embryos, highlighting areas of reprogramming resistance.
Area of Science:
- Reproductive biology
- Genomics
- Developmental biology
Background:
- Incomplete reprogramming in somatic cell nuclear transfer (SCNT) embryos causes gene misregulation, leading to developmental issues and lethality.
- Understanding these reprogramming errors is crucial for improving cloning efficiency.
Purpose of the Study:
- To compare transcriptome profiles of bovine blastocysts produced by in vitro fertilization (IVF) and SCNT using different donor cells (cumulus and fibroblast).
- To identify differentially expressed genes and reprogramming-resistant regions in SCNT blastocysts.
Main Methods:
- Single-cell transcriptome profiling of bovine blastocysts from IVF, cumulus cell-derived SCNT (cSCNT), and fibroblast-derived SCNT (fSCNT).
- Differential gene expression analysis and correlation analysis of transcriptomic profiles.
- Identification of specific genomic loci with altered gene expression.
Main Results:
- SCNT blastocysts showed transcriptomic profiles distinct from IVF blastocysts, with cSCNT closer to IVF than fSCNT.
- Identified 56 underrepresented and 78 overrepresented differentially expressed genes in SCNT embryos.
- A 400-kb region on chromosome 18 with zinc-finger protein genes was coordinately down-regulated in fSCNT, indicating a reprogramming-resistant locus.
Conclusions:
- SCNT efficiency is influenced by donor cell type and results in specific gene expression errors.
- Genes involved in trophectoderm development were often underrepresented, while epigenetic modifiers were overrepresented in SCNT blastocysts.
- Mapping reprogramming-resistant loci can help improve SCNT efficiency by addressing stochastic reprogramming events.
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