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Updated: Apr 4, 2026
Bioluminescent Optogenetics 2.0: Harnessing Bioluminescence to Activate Photosensory Proteins In Vitro and In Vivo
Published on: August 4, 2021
A two-photon activatable amino acid linker for the induction of fluorescence
Felix Friedrich1, Kathrin Klehs, Manuela A H Fichte
1Goethe University Frankfurt, Institute for Organic Chemistry and Chemical Biology, Max-von-Laue-Str. 9, 60438 Frankfurt, Germany. heckel@uni-frankfurt.de.
Abstract:
A new one- and two-photon activatable fluorophore based on ATTO565 was developed using a photolabile linker that simultaneously acts as a quencher. It is especially interesting for protein and peptide applications because it can be incorporated by standard peptide chemistry. The application of the new fluorogenic construct in super-resolution microscopy of antibody conjugates is shown.
