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Expansion of Two-dimension Electrospun Nanofiber Mats into Three-dimension Scaffolds
Published on: January 7, 2019
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Cellulose acetate based 3-dimensional electrospun scaffolds for skin tissue engineering applications.
Deniz Atila1, Dilek Keskin2, Ayşen Tezcaner2
1Department of Engineering Sciences, Middle East Technical University, Turkey.
Carbohydrate Polymers
|September 8, 2015
Summary
Researchers developed novel 3D cellulose acetate (CA) and pullulan (PULL) scaffolds for skin tissue engineering. These biocompatible scaffolds promote cell growth and show potential for healing skin defects.
Area of Science:
- Biomaterials Science
- Regenerative Medicine
- Tissue Engineering
Background:
- Skin defects pose significant clinical challenges due to limited self-regeneration capabilities.
- Tissue engineering offers a promising therapeutic strategy for addressing these defects.
- Developing advanced scaffolds that mimic native tissue is crucial for promoting wound healing.
Purpose of the Study:
- To develop and characterize novel 3-dimensional (3D) electrospun scaffolds using cellulose acetate (CA) and pullulan (PULL) for skin tissue engineering.
- To investigate the influence of varying CA/PULL ratios on scaffold properties.
- To evaluate the cytocompatibility of the developed scaffolds for potential skin regeneration applications.
Main Methods:
- Fabrication of 3D electrospun CA/PULL scaffolds with adjustable heights using pullulan as a porogen.
- Characterization of scaffolds including porosity, degradation, and mechanical properties at different polymer ratios.
- Assessment of scaffold suitability as cell carriers after pullulan removal.
- In vitro cell culture studies using L929 mouse fibroblastic cells to evaluate cell adhesion, proliferation, and population.
Main Results:
- Scaffold fiber diameter, thickness, and porosity increased with higher pullulan content, leading to increased degradation.
- Mechanical strength of scaffolds improved significantly after pullulan removal, indicating suitability as cell carriers.
- In vitro studies demonstrated excellent cytocompatibility, with L929 cells adhering, proliferating, and populating the CA/PULL (50/50) scaffolds.
Conclusions:
- Uncrosslinked CA/PULL (50/50) electrospun scaffolds are cytocompatible and possess suitable properties for skin tissue engineering.
- The developed 3D scaffolds show significant potential for promoting the healing of skin defects.
- This study presents a novel approach for creating advanced biomaterials for regenerative medicine applications.
