[Cytogenetic study on a human colorectal carcinoma cell line (HR-8348)]

Cytogenetics of a human colorectal carcinoma cell line (HR-8348) established in China were described. The early (29th passage) and late passages (93th passage) of this cell line were used for chromosome analysis. HR-8348 was a cell line essentially with near triploid karyotype. The distribution of chromosome number was rather dispersed in the early passage, whereas it was concentrated to 65-70 range in the late passage. G-banding stain showed that the numerical distribution of different chromosomes was also dispersed in the early passage associated with abundant abnormal chromosomes. In the late passage, it turned to be more stable and the number of abnormal chromosomes reduced. In 60 metaphases analysed, 10 marker chromosomes were found. The frequency of M1, M3 and M2 was 100% in both early and late passages. The morphologic characteristics and their possible origin and role in the pathogenesis of colorectal carcinoma were discussed.