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Updated: Apr 4, 2026
Expansion of Two-dimension Electrospun Nanofiber Mats into Three-dimension Scaffolds
Published on: January 7, 2019
A three-dimensional hierarchical scaffold fabricated by a combined rapid prototyping technique and electrospinning
Yun Gyeong Kang1, Ji Won Shin1, So Hee Park1
1Department of Biomedical Engineering, Inje University, Rm #309, BLDG-A, 607 Obang-Dong, Gimhae, Gyeongnam, 621-749, Republic of Korea.
Objective:
To investigate the expansion of hematopoietic stem/progenitor cells (HSPCs) from umbilical cord blood using extracellular matrix (ECM) protein-coated three-dimensional hierarchical scaffolds.
Results:
The expansion of HSPCs was evaluated through total nucleated cell (TNC) expansion, immuno-phenotypic analysis, and clonogenic ability. After 7 days of culture, three-dimensional cultures with fibronectin-coated scaffolds achieved the highest fold increase in TNCs (164 ± 6.9 fold) and the highest CD45(+)CD34(+) (35 %) and CD34(+)CD38(-) (32 %) ratios.
Conclusion:
Three-dimensional hierarchical scaffolds were coated with ECM protein to simulate a biomimetic environment or niche, and had a significant effect on the expansion potential of HSPCs without changing their phenotype.
