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Updated: Apr 4, 2026
Use of Label-free Optical Biosensors to Detect Modulation of Potassium Channels by G-protein Coupled Receptors
Published on: February 10, 2014
Optical birefringence of liquid crystals for label-free optical biosensing diagnosis
Tan Tai Nguyen1, Gyeo-Re Han2, Chang-Hyun Jang3
1Department of Bionano Technology, Gachon University, Seongnam-City, South Korea.
Purpose:
We present a polarization-sensitive optical detection platform for label-free quantitative optical biosensing diagnosis using liquid crystals (LCs). This is capable of determining quantitatively the optical birefringence of optical cells containing LCs, whose orientation depends on the immobilized biomolecules.
Patients And Methods:
This technique uses a polarization-dependent double-port detection without any polarizer at a single wavelength and removes the need of aligning optical cells of LCs in the azimuthal direction, with respect to the light path through the optical cell. Thus, this technique enables a stand-alone detection in a relatively compact format without an additional optical instrument, such as a retardation compensator, a Michael-Levy chart, and a spectrophotometer, in order to determine the optical birefringence quantitatively.
Results:
We demonstrate that bovine serum albumin immobilized on the gold surface of the cell hybrid interfaces that support both homeotropic and planar anchoring of LCs causes optical phase retardation change which can be determined quantitatively. We also provide estimation of the zenithal orientation of LCs near the gold surface of the hybrid interfaces, based on the phase retardation determined. The estimated limit of bovine serum albumin detection is approximately 2.1 μM.
Conclusion:
This optical technique with LCs can serve an optical platform for label-free quantitative diagnosis of proteins in a real time manner.
