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Updated: Apr 4, 2026

Dopamine Release at Individual Presynaptic Terminals Visualized with FFNs
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Visualizing dopamine released from living cells using a nanoplasmonic probe.

W W Qin1, S P Wang, J Li

  • 1Division of Physical Biology & Bioimaging Centre, Shanghai Synchrotron Radiation Facility, CAS Key Laboratory of Interfacial Physics and Technology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China. lidi@sinap.ac.cn.

Nanoscale
|September 9, 2015
PubMed
Summary
This summary is machine-generated.

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We developed a sensitive nanoplasmonic probe for detecting dopamine release from living cells. This new method visualizes dopamine release and its link to calcium influx in real-time.

Area of Science:

  • Biomedical Engineering
  • Nanotechnology
  • Neuroscience

Background:

  • Dopamine is a crucial neurotransmitter involved in various physiological processes.
  • Accurate detection and imaging of dopamine release are essential for understanding neurological functions and diseases.

Purpose of the Study:

  • To develop an ultrasensitive nanoplasmonic probe for discriminative detection and imaging of dopamine.
  • To investigate the mechanism of dopamine release from living cells stimulated by adenosine triphosphate (ATP).

Main Methods:

  • Utilized dopamine-induced seeded-growth of gold nanoparticles (Au NPs) for sensing.
  • Employed dark-field microscopy (DFM) to visualize dopamine release from rat pheochromocytoma (PC12) cells.
  • Integrated real-time fluorescence imaging of intracellular calcium (Ca2+) dynamics.

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Related Experiment Videos

Last Updated: Apr 4, 2026

Dopamine Release at Individual Presynaptic Terminals Visualized with FFNs
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Gold Nanorod-assisted Optical Stimulation of Neuronal Cells
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Main Results:

  • Achieved dopamine detection limit as low as 0.25 pM within 1 minute.
  • Visualized ATP-stimulated dopamine release from PC12 cells.
  • Demonstrated that dopamine release is concomitant with Ca2+ influx via ATP-activated channels, not voltage-gated channels.

Conclusions:

  • The developed nanoplasmonic probe offers a highly sensitive and rapid method for dopamine detection and imaging.
  • The study elucidates the role of ATP-activated channels in dopamine release linked to Ca2+ influx in PC12 cells.