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Multifunctional, Micropipette-based Method for Incorporation And Stimulation of Bacterial Mechanosensitive Ion Channels in Droplet Interface Bilayers

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Published on: November 19, 2015

Cell-Instructive Microgels with Tailor-Made Physicochemical Properties.

Simone Allazetta¹, Laura Kolb¹, Samantha Zerbib¹

¹Laboratory of Stem Cell Bioengineering, Institute of Bioengineering, School of Life Sciences and School of Engineering, Ecole Polytechnique Fédérale de Lausanne (EPFL), 1015, Lausanne, Switzerland.

Small (Weinheim an Der Bergstrasse, Germany)

|September 10, 2015

Summary

This summary is machine-generated.

A novel microfluidic platform enables systematic testing of 3D microenvironmental effects on cell fate using tunable microgels. This technology enhances cell encapsulation for studying cell viability and morphology in various conditions.

Keywords:

3D encapsulation hydrogels microfluidics microgels stem cells

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Light-mediated Formation and Patterning of Hydrogels for Cell Culture Applications

Light-mediated Formation and Patterning of Hydrogels for Cell Culture Applications

Published on: September 29, 2016

Area of Science:

Biotechnology
Cell Biology
Materials Science

Background:

Understanding cell fate in 3D requires controlled microenvironments.
Existing methods lack systematic control over microenvironmental parameters.

Purpose of the Study:

Develop a microfluidic platform for in vitro cell encapsulation.
Systematically investigate microenvironmental parameter effects on cell fate in 3D.

Main Methods:

Utilized microfluidics to create enzymatically cross-linked poly(ethylene glycol)-based microgels.
Incorporated fibroblasts, embryonic stem cells, and cancer cells.
Explored methods to prevent cell escape from microcapsules and coencapsulated microgels within nondegradable gels.

Main Results:

Demonstrated tunable mechanical and biochemical properties of microgels.
Showcased enhanced cell encapsulation efficiency through optimized methods.
Successfully maintained cell viability, proliferation, and morphology for up to two weeks in culture under diverse microenvironmental conditions.

Conclusions:

The developed microfluidic platform offers a powerful tool for 3D cell fate studies.
Tunable microgels and improved encapsulation enhance the potential for in vitro cell microenvironment research.
This technology facilitates long-term studies of cell behavior in controlled 3D settings.