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Updated: Apr 4, 2026

Generation of Scalable, Metallic High-Aspect Ratio Nanocomposites in a Biological Liquid Medium

Generation of Scalable, Metallic High-Aspect Ratio Nanocomposites in a Biological Liquid Medium

Published on: July 8, 2015

Progress toward clonable inorganic nanoparticles.

Thomas W Ni¹, Lucian C Staicu, Richard S Nemeth

¹Department of Chemistry, Colorado State University, Fort Collins, CO 80523, USA. ackerson@colostate.edu.

|September 10, 2015

Summary

This summary is machine-generated.

This study identifies glutathione reductase as a key enzyme produced by Pseudomonas moraviensis stanleyae for creating selenium nanoparticles. The enzyme facilitates selenite reduction and nanoparticle formation within the bacterium.

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Last Updated: Apr 4, 2026

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A Continuous-flow Photocatalytic Reactor for the Precisely Controlled Deposition of Metallic Nanoparticles

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Area of Science:

Microbiology
Biotechnology
Environmental Science

Background:

Pseudomonas moraviensis stanleyae, isolated from the selenium hyperaccumulator plant Stanleya pinnata, tolerates high selenium concentrations.
This bacterium produces intracellular selenium (Se) nanoparticles.

Purpose of the Study:

To identify the enzymes responsible for selenite reduction and Se nanoparticle formation in P. moraviensis stanleyae.
To characterize the Se nanoparticles and their formation process.

Main Methods:

Cellular electron tomography for nanoparticle structure analysis.
Protein mass spectrometry to identify candidate enzymes.
In vitro experiments with purified glutathione reductase.

Main Results:

Glutathione reductase homologues were identified as candidate enzymes for selenite reduction.
In vitro assays confirmed NADPH-dependent reduction of selenite to Se nanoparticles by glutathione reductase.
Nanoparticle size correlated with selenite concentration (5 nm at 1.0 μM to 50 nm at 100 μM).
Glutathione reductase appears to be retained within or entombed by the formed nanoparticles.

Conclusions:

Glutathione reductase is a key enzyme for bacterial Se nanoparticle biogenesis.
The enzyme exhibits characteristics of a self-clonable nanoparticle system, including ion reduction, retention, and size control.