Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Protein Dynamics in Living Cells

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

[Mechanisms of gut<b>-</b>derived trimethylamine<b>-</b>N<b>-</b>oxide in mediating multiple organ injury in post<b>-</b>cardiac arrest syndrome].

Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. Medical sciences·2026

Same author

The influence of pterygium size on corneal refractive properties and higher-order aberrations.

BMC ophthalmology·2026

Same author

Neuroprotective Effects of Glucagon-like Peptide-1/glucagon-like Peptide-1 Receptor Agonists on Neurodegenerative Eye Diseases and their Molecular Mechanisms: A Recent Update.

Current neuropharmacology·2026

Same author

Pepper Veinal Mottle Virus (PVMV)-Based Vector for Expressing Pathogen-Derived Resistance in Pepper.

Methods in molecular biology (Clifton, N.J.)·2026

Same author

Mild Strain Cross-Protection in Passion Fruit.

Methods in molecular biology (Clifton, N.J.)·2026

Same author

MSCNet: Efficient and accurate semantic segmentation of LiDAR data using Multi-scale Convolution.

PloS one·2026

Search research articles

Related Experiment Video

Updated: Apr 4, 2026

Fluorescent End-Labeling and Encapsulation of Long RNAs for Single-Molecule FRET-TIRF Microscopy

Fluorescent End-Labeling and Encapsulation of Long RNAs for Single-Molecule FRET-TIRF Microscopy

Published on: October 18, 2024

Visualizing double-stranded RNA distribution and dynamics in living cells by dsRNA binding-dependent fluorescence

Xiaofei Cheng¹, Ping Deng², Hongguang Cui²

¹Southern Crop Protection and Food Research Centre, Agriculture and Agri-Food Canada, London, Ontario N5V 4T3, Canada; College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, Zhejiang 310036, PR China.

|September 10, 2015

Summary

Scientists developed a new method to visualize double-stranded RNA (dsRNA) in living cells. This dsRNA binding-dependent fluorescence complementation (dRBFC) assay tracks viral RNA dynamics in real-time.

Keywords:

BiFC D-bodies Double-stranded RNA Fluorescent protein Live-cell imaging Positive-sense RNA virus RDR6 RNA binding protein SGS3 Viral replication complex

More Related Videos

Real-time Imaging of Single Engineered RNA Transcripts in Living Cells Using Ratiometric Bimolecular Beacons

Real-time Imaging of Single Engineered RNA Transcripts in Living Cells Using Ratiometric Bimolecular Beacons

Published on: August 6, 2014

Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells

Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells

Published on: December 9, 2022

Related Experiment Videos

Last Updated: Apr 4, 2026

Fluorescent End-Labeling and Encapsulation of Long RNAs for Single-Molecule FRET-TIRF Microscopy

Fluorescent End-Labeling and Encapsulation of Long RNAs for Single-Molecule FRET-TIRF Microscopy

Published on: October 18, 2024

Real-time Imaging of Single Engineered RNA Transcripts in Living Cells Using Ratiometric Bimolecular Beacons

Real-time Imaging of Single Engineered RNA Transcripts in Living Cells Using Ratiometric Bimolecular Beacons

Published on: August 6, 2014

Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells

Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells

Published on: December 9, 2022

Area of Science:

Molecular Biology
Virology
Cell Biology

Background:

Double-stranded RNA (dsRNA) is crucial for eukaryotic cellular processes and a key indicator of positive-sense RNA virus infections.
Existing technologies lack the capability for in vivo visualization of dsRNA within living cells.

Purpose of the Study:

To develop and validate a novel in vivo technology for monitoring dsRNA distribution and dynamics.
To enable real-time visualization of viral RNA replication intermediates.

Main Methods:

Developed a dsRNA binding-dependent fluorescence complementation (dRBFC) assay.
Engineered fusion proteins comprising dsRNA-binding domains and split yellow fluorescent protein (YFP) halves.
Utilized YFP reconstitution upon dsRNA binding to signal the presence of dsRNA.

Main Results:

Successfully visualized dsRNA distribution and dynamics in living cells using the dRBFC assay.
Demonstrated the system's ability to monitor the replicative RNA intermediates of positive-sense RNA viruses.
Confirmed that binding of fusion proteins to dsRNA restores YFP fluorescence.

Conclusions:

The dRBFC assay provides an efficient tool for in vivo dsRNA visualization.
This technology facilitates the study of viral RNA replication and cellular dsRNA roles.
Enables real-time tracking of dsRNA in living systems.