Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

14.8K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
14.8K
Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

3.0K
Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
3.0K
Three-Dimensional Microscopy in Microbiology01:28

Three-Dimensional Microscopy in Microbiology

1.0K
Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...
1.0K
Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

15.2K
Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
15.2K
Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

13.7K
Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
13.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Optical sectioning in wide-field two-photon microscopy using temporal focusing and random illumination.

Optics letters·2026
Same author

Accuracy of the multilayer Born approximation in transmission and reflection.

Journal of the Optical Society of America. A, Optics, image science, and vision·2025
Same author

Transparent Single-Layer Graphene Electrodes for Deciphering and Enhancing Microbead-Based Electrochemiluminescence Immunoassay.

Small (Weinheim an der Bergstrasse, Germany)·2025
Same author

Comment on "Fast and accurate electromagnetic field calculation for substrate-supported metasurfaces using the discrete dipole approximation".

Nanophotonics (Berlin, Germany)·2024
Same author

Quantitative phase microscopies: accuracy comparison.

Light, science & applications·2024
Same author

Extended-depth of field random illumination microscopy, EDF-RIM, provides super-resolved projective imaging.

Light, science & applications·2024
Same journal

Multi-module collaborative optimization-driven fast speckle correlation imaging in variable environments.

Journal of the Optical Society of America. A, Optics, image science, and vision·2026
Same journal

Secrecy performance analysis of NOMA-UWOC systems over a vertically stratified WGG oceanic turbulence channel.

Journal of the Optical Society of America. A, Optics, image science, and vision·2026
Same journal

Backscattering of plane waves in a composite system containing a rough surface and anisotropic scatterers.

Journal of the Optical Society of America. A, Optics, image science, and vision·2026
Same journal

Aspherical surface construction methods based on extended Jacobi polynomials.

Journal of the Optical Society of America. A, Optics, image science, and vision·2026
Same journal

OCT sidelobe suppression method based on dual-path phase sinusoidal modulation and minimum value fusion.

Journal of the Optical Society of America. A, Optics, image science, and vision·2026
Same journal

Optical design concepts using wavelength-selective diffractive optics to enable miniaturized multimodal endoscopic imaging across separated spectral ranges.

Journal of the Optical Society of America. A, Optics, image science, and vision·2026
See all related articles

Related Experiment Video

Updated: Apr 3, 2026

Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution
08:41

Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution

Published on: August 16, 2012

12.1K

Superresolution with full-polarized tomographic diffractive microscopy.

Charankumar Godavarthi, Ting Zhang, Guillaume Maire

    Journal of the Optical Society of America. A, Optics, Image Science, and Vision
    |September 15, 2015
    PubMed
    Summary
    This summary is machine-generated.

    Polarization-resolved measurements significantly enhance the accuracy and resolution of tomographic diffractive microscopy. This advanced technique achieves approximately 100 nm isotropic transverse resolution for 3D imaging.

    More Related Videos

    Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers
    10:07

    Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers

    Published on: April 9, 2014

    10.7K
    Author Spotlight: Non-Invasive Imaging of Complex Bio-Structures Using Polarization-Sensitive Two-Photon Microscopy
    05:54

    Author Spotlight: Non-Invasive Imaging of Complex Bio-Structures Using Polarization-Sensitive Two-Photon Microscopy

    Published on: September 8, 2023

    2.0K

    Related Experiment Videos

    Last Updated: Apr 3, 2026

    Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution
    08:41

    Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution

    Published on: August 16, 2012

    12.1K
    Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers
    10:07

    Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers

    Published on: April 9, 2014

    10.7K
    Author Spotlight: Non-Invasive Imaging of Complex Bio-Structures Using Polarization-Sensitive Two-Photon Microscopy
    05:54

    Author Spotlight: Non-Invasive Imaging of Complex Bio-Structures Using Polarization-Sensitive Two-Photon Microscopy

    Published on: September 8, 2023

    2.0K

    Area of Science:

    • Optics and Photonics
    • Materials Science
    • Biomedical Imaging

    Background:

    • Tomographic diffractive microscopy (TDM) reconstructs 3D permittivity maps from scattered light.
    • Conventional scalar treatments in TDM have limitations in accuracy and resolution.
    • Polarization information in scattered light is often underutilized.

    Purpose of the Study:

    • To investigate the impact of polarization-resolved measurements on TDM.
    • To improve the accuracy and resolution of 3D permittivity reconstructions.
    • To demonstrate sub-100 nm transverse resolution using TDM.

    Main Methods:

    • Implemented polarization-resolved detection in TDM.
    • Acquired scattered field data with phase and amplitude information.
    • Reconstructed 3D permittivity maps using the enhanced data.

    Main Results:

    • Polarization-resolved TDM significantly improved reconstruction accuracy.
    • Achieved an isotropic transverse resolution of approximately 100 nm at 475 nm wavelength.
    • Demonstrated superior performance compared to scalar TDM methods.

    Conclusions:

    • Polarization-resolved measurements are crucial for high-resolution TDM.
    • This method offers a pathway to advanced 3D nanoscale imaging.
    • The technique has potential applications in materials science and biomedical imaging.