Flow-through and catheter biosensors with an extended concentration range

Area of Science:

• Biomedical Engineering
• Analytical Chemistry
• Biosensor Technology

Background:

• Continuous monitoring of blood metabolites is crucial for managing various health conditions.
• Existing biosensors may face limitations in linearity or applicability across diverse analytes.
• Catheter-based sensors offer potential for minimally invasive, in-situ measurements.

Purpose of the Study:

• To design and develop novel flow-through and catheter-based biosensors.
• To achieve linear calibration curves for key blood metabolites within their physiological concentration ranges.
• To enable simultaneous or individual detection of glucose, lactate, ethanol, and uric acid.

Main Methods:

• Fabrication of specialized electrodes for electrochemical detection.
• Immobilization of specific enzymes or mediators onto sensor surfaces.
• Testing sensor performance in buffer solutions and biological samples.
• Validation of calibration curves across physiological concentration ranges.

Main Results:

• Designed flow-through and catheter biosensors for targeted analytes.
• Demonstrated linear calibration curves for glucose, lactate, ethanol, and uric acid sensors.
• Achieved linearity within the physiological concentration range of these metabolites in blood.
• Confirmed sensor functionality for multiple metabolite detection.

Conclusions:

• The developed catheter biosensors provide a reliable platform for monitoring key blood metabolites.
• Linearity across physiological ranges ensures accurate quantification for clinical applications.
• These biosensors hold promise for improved diagnostics and therapeutic management.