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Altered Plasma MicroRNAs as Novel Biomarkers for Arteriosclerosis Obliterans
Xue-Mei He1, Ying-Qiang Zheng, Sheng-Zhi Liu
1Experimental Medicine Center, Affiliated Hospital of Luzhou Medical College.
Aim:
Arteriosclerosis obliterans (ASO) of the lower extremities is a major cause of adult limb loss worldwide. A timely diagnosis in the early stages of the disease determines the clinical outcomes, however lacking of palpable symptoms remains the biggest obstacle. This study aimed to screen a cluster of microRNAs (miRNAs) that can be used as biomarker for the ASO in the earlier stages.
Methods:
Plasma from 3 patients with ASO and 3 healthy controls were profiled to screen altered miRNAs by microarray, then Real time PCR was further used to confirm the changes in 55 ASO patients and 54 controls.We also analyzed the correlation of miRNAs level with Fontaine stages and the influence of T2DM which is a common complication with ASO on the level of miRNAs.
Result:
Twenty-four aberrantly expressed miRNAs were screened in the plasma of ASO patients. Real time PCR verified that the level of miR-4284 was significantly increased, while levels of miR-4463, miR-4306 and miR-221-3p were significantly decreased both in the plasma and in the sclerotic samples compared with the controls. Interestingly, we revealed a time and stage specific expression manner, as shown that expression of miR-4284 increased at the stage I of ASO and maintained the tendency to stage IV, while miR-4463 expression decreased at every stage of ASO; however, the expression of miR-4463 showed opposite changes in ASO patients with or without T2DM.
Conclusion:
Altered expressions of miR-4284 and miR-4463 are novel characteristics and may serve as potential biomarkers for the early diagnosis of ASO.
Insights
Early diagnosis of lower extremity arteriosclerosis obliterans (ASO) is crucial. This study identified specific microRNAs (miRNAs) in plasma as potential early biomarkers for ASO, aiding timely intervention and preventing limb loss.
Area of Science:
- Biochemistry
- Molecular Biology
- Vascular Medicine
Background:
- Arteriosclerosis obliterans (ASO) of the lower extremities is a leading cause of limb loss.
- Early diagnosis is critical for effective treatment but is hindered by a lack of early symptoms.
- MicroRNAs (miRNAs) are being investigated as potential diagnostic biomarkers.
Purpose of the Study:
- To screen for microRNAs (miRNAs) that can serve as early diagnostic biomarkers for arteriosclerosis obliterans (ASO).
- To identify specific miRNAs in plasma that are altered in the early stages of ASO.
Main Methods:
- Plasma samples from ASO patients and healthy controls were analyzed using microarray and quantitative real-time PCR.
- Expression levels of selected miRNAs were correlated with disease severity (Fontaine stages) and the presence of type 2 diabetes mellitus (T2DM).
Main Results:
- Twenty-four miRNAs showed altered expression in ASO patients.
- miR-4284 levels were significantly increased, while miR-4463, miR-4306, and miR-221-3p levels were decreased in ASO patients.
- miR-4284 expression increased from stage I to IV, while miR-4463 decreased across all stages, with differing expression patterns in patients with T2DM.
Conclusions:
- Altered expression of miR-4284 and miR-4463 are potential novel biomarkers for the early detection of ASO.
- These miRNAs could facilitate timely diagnosis and intervention, potentially improving clinical outcomes for ASO patients.
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