[Detection of HIV antibody and antigen]

A total of 75 specimens (45 sera, 17 CSF and 13 stool) was collected from 65 participants (39 AIDS, 14 high risk group and 12 laboratory personnel) and HIV antibody and antigen were detected. Twenty sera and 16 CSF submitted by AIDS patients showed positive antibody reactions in both ELISA and Western blot assay, whereas, antibody was not detected in the sera and the CSF obtained from high risk group or laboratory personnel. An overall of 21% (10/48) positive rate was found in the specimens submitted by AIDS patients for HIV antigen detection by ELISA. The positive rates of HIV antigen in sera, CSF and stool specimen from the AIDS patients were 30% (6/20), 13% (2/16) and 17% (2/12), respectively.