Related Experiment Video
Updated: Apr 3, 2026

An Alternative Culture Method to Maintain Genomic Hypomethylation of Mouse Embryonic Stem Cells Using MEK Inhibitor PD0325901 and Vitamin C
Published on: June 1, 2018
Thermoresponsive hydrogel maintains the mouse embryonic stem cell "naïve" pluripotency phenotype
Christian Mangani1, Annamaria Lilienkampf, Marcia Roy
1School of Chemistry, EaStCHEM, University of Edinburgh, Joseph Black Building, West Mains Road, Edinburgh, EH9 3FJ, UK. Mark.Bradley@ed.ac.uk.
Abstract:
A chemically defined thermoresponsive hydrogel, poly(AEtMA-Cl-co-DEAEA) cross-linked with N,N'-methylenebisacrylamide, which allows enzyme-free passaging, was used as a substrate to culture murine embryonic stem cells (mESCs) under defined and undefined conditions. Analysis of 14 stem cell markers showed that the mESCs remained in a "naïve" state of pluripotency with differentiation potential to form endoderm, mesoderm, and ectoderm derived lineages. These results validate the use of a chemically defined hydrogel for standardised and inexpensive mESC culture.
More Related Videos
09:34Reprogramming Primary Amniotic Fluid and Membrane Cells to Pluripotency in Xeno-free Conditions
Published on: November 27, 2017
11:37Human Pluripotent Stem Cell Culture on Polyvinyl Alcohol-Co-Itaconic Acid Hydrogels with Varying Stiffness Under Xeno-Free Conditions
Published on: February 3, 2018