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Rotavirus disrupts cytoplasmic P bodies during infection

Rahul Bhowmick1, Arpita Mukherjee1, Upayan Patra1

  • 1Division of Virology, National Institute of Cholera and Enteric Diseases, P-33, C.I.T. Road, Scheme-XM, Beliaghata, Kolkata 700010, West Bengal, India.

Virus Research
|September 20, 2015
PubMed

Insights

Rotavirus infection disrupts cellular Processing bodies (P bodies) by degrading key components and relocating others to the nucleus. This viral strategy helps rotavirus evade the host

Area of Science:

  • Virology
  • Cell Biology
  • Molecular Biology

Background:

  • Cytoplasmic Processing bodies (P bodies) are crucial sites for mRNA regulation, including decay.
  • Viruses are known to manipulate P bodies to facilitate their replication.
  • Rotavirus, a common cause of infantile gastroenteritis, replicates in the host cell cytoplasm.

Purpose of the Study:

  • To investigate the impact of rotavirus infection on the integrity and function of P bodies.
  • To elucidate the mechanisms by which rotavirus affects P bodies.

Main Methods:

  • Confocal microscopy to visualize P body components.
  • Western blotting to assess protein degradation.
  • Subcellular fractionation to determine protein localization.

Main Results:

  • Rotavirus infection leads to the disruption of P bodies.
  • The P body component Pan3 is degraded by rotavirus non-structural protein 1 (NSP1).
  • Exonuclease XRN1 and decapping enzyme hDCP1a are relocalized from the cytoplasm to the nucleus.

Conclusions:

  • Rotavirus employs novel strategies involving protein degradation and subcellular relocalization to disrupt P bodies.
  • These mechanisms contribute to the subversion of cellular antiviral responses by rotavirus.
  • Understanding these interactions can reveal new targets for antiviral therapies.
Keywords:
DegradationHost–virus interactionNuclear translocationP-BodyRotavirus

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