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Point-, line-, and plane-shaped cellular constructs for 3D tissue assembly.

Yuya Morimoto1, Amy Y Hsiao1, Shoji Takeuchi1

  • 1Center for International Research on Integrative Biomedical Systems (CIBiS), Institute of Industrial Science (IIS), The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8505, Japan; Takeuchi Biohybrid Innovation Project, Exploratory Research for Advanced Technology (ERATO), Japan Science and Technology (JST), Komaba Open Laboratory (KOL) Room M202, 4-6-1, Komaba, Meguro-ku, Tokyo 153-8904, Japan.

Advanced Drug Delivery Reviews
|September 22, 2015
PubMed
Summary
This summary is machine-generated.

Microfluidic techniques enable the creation of precise, microsized cellular constructs for bottom-up tissue engineering. These building blocks can be assembled into larger tissues for biological studies and clinical applications.

Keywords:
3D tissue fabricationBottom-up tissue engineeringCellular building blocksClinical treatmentsMicrofluidic techniques

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Area of Science:

  • Biotechnology
  • Tissue Engineering
  • Microfluidics

Background:

  • Microsized cellular constructs, including aggregates and hydrogel blocks, are key for reconstructing 3D tissues.
  • Bottom-up tissue engineering relies on spatially ordered cells within these constructs.

Purpose of the Study:

  • To review microfluidic methods for forming microsized cellular constructs.
  • To introduce techniques for assembling these constructs into larger, arbitrary-shaped tissues.
  • To explore applications in biological studies and clinical treatments.

Main Methods:

  • Utilizing microfluidic techniques for high-throughput formation of cellular constructs.
  • Controlling construct shapes (point, line, plane) for precise fabrication.
  • Developing manipulation techniques for controlled assembly of constructs.

Main Results:

  • Demonstrated the ability of microfluidics to produce diverse microsized cellular constructs.
  • Showcased assembly methods for creating complex tissue architectures.
  • Highlighted the versatility of these constructs for various applications.

Conclusions:

  • Microfluidic-based microsized cellular constructs are vital for advanced tissue engineering.
  • Assembly of these constructs offers a promising route for fabricating functional tissues.
  • Future applications span biological research and clinical therapeutic strategies.