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Selective Binding of DNA Origami on Biomimetic Lipid Patches

Michael Hirtz1, Josipa Brglez2, Harald Fuchs1,3

  • 1Institute of Nanotechnology (INT) and Karlsruhe Nano Micro Facility (KNMF), Karlsruhe Institute of Technology (KIT), 76128, Karlsruhe, Germany.

Small (Weinheim an Der Bergstrasse, Germany)
|September 22, 2015
PubMed

Keywords:
DNA origamibiomimetic membranesdip-pen nanolithographyphospholipidssupported lipid bilayer membranes

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For successful DNA replication, the unwinding of double-stranded DNA must be accompanied by stabilization and protection of the separated single strands of the DNA. This crucial task is performed by single-strand DNA-binding (SSB) proteins. They bind to the DNA in a sequence-independent manner, which means that the nitrogenous bases of the DNA need not be present in a specific order for binding of SSB proteins to it. The binding of SSB proteins straightens single-stranded DNA (ssDNA) and makes...