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Romanowsky staining using liquid-based cytology: A pilot study using Cytolyt(®)/HESPANDER(®) processing solution for

Yuichi Kinoshita^1,2, Takashi Yuri¹, Katsuhiko Yoshizawa¹

¹Department of Pathology II, Kansai Medical University, Hirakata, Osaka, Japan.

Diagnostic Cytopathology

|September 22, 2015

Summary

This summary is machine-generated.

Romanowsky May-Grünwald-Giemsa (MGG) staining was evaluated for liquid-based cytology (LBC) using the ThinPrep(®) method. This MGG-LBC technique provides clear diagnostic details for various cell types and conditions.

Keywords:

CytoLyt/HESPANDER solution Romanowsky stain liquid-based cytology pleural effusion urine

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Area of Science:

Cytopathology
Histology
Biomedical diagnostics

Background:

Liquid-based cytology (LBC) is a widely used sample preparation technique.
Romanowsky stains have not been previously evaluated for LBC.
A novel technique, May-Grünwald-Giemsa staining with ThinPrep(®) (MGG-LBC), is introduced.

Purpose of the Study:

To evaluate the efficacy of Romanowsky May-Grünwald-Giemsa (MGG) staining in liquid-based cytology (LBC).
To assess the diagnostic utility of MGG-LBC preparations for various clinical samples.

Main Methods:

KPL-1 breast cancer cells were prepared using conventional smear and MGG-LBC methods.
Cell size, nucleus/cytoplasm (N/C) ratio, and morphology were analyzed.
Clinical samples (urine, pleural effusion) were examined using MGG-LBC.

Main Results:

MGG-LBC showed lower cellularity than Papanicolaou-stained ThinPrep(®) smears.
Cell size and N/C ratio trends were similar between MGG-LBC and conventional methods.
MGG-LBC provided clear visualization of reactive mesothelial cells, urothelial cells, carcinoma cells, crystals, and bacteria.

Conclusions:

Romanowsky staining is crucial for specific cytodiagnostic applications.
MGG-LBC can enhance cytodiagnostic accuracy in LBC preparations.