Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Integrating Rapid Cardiopulmonary and Gastric Ultrasound for Emergency Airway Management in Critically Ill Patients: A Case Series of Resident-Performed Echocardiographic Assessment Using Subcostal-Only-View in Physiologically Difficult Airway.

Critical care explorations·2025
Same author

Glycoform analysis of recombinant and human immunodeficiency virus envelope protein gp120 via higher energy collisional dissociation and spectral-aligning strategy.

Analytical chemistry·2014
Same author

Bipolar radiofrequency ablation of liver tumors: technical experience and interval follow-up in 22 patients with 33 ablations.

Journal of surgical oncology·2012
Same author

Prospective randomized comparison of onabotulinumtoxinA (Botox) and abobotulinumtoxinA (Dysport) in the treatment of forehead, glabellar, and periorbital wrinkles.

Aesthetic surgery journal·2012
Same author

[Inhibitory effects of Qushi Huayu Decoction on fatty deposition and tumor necrosis factor alpha secretion in HepG2 cells induced by free fatty acid].

Zhongguo Zhong xi yi jie he za zhi Zhongguo Zhongxiyi jiehe zazhi = Chinese journal of integrated traditional and Western medicine·2008
Same author

Bioactive polybrominated diphenyl ethers from the marine sponge Dysidea sp.

Journal of natural products·2008

Related Experiment Video

Updated: Apr 3, 2026

A Quantitative Glycomics and Proteomics Combined Purification Strategy
11:38

A Quantitative Glycomics and Proteomics Combined Purification Strategy

Published on: March 8, 2016

15.6K

Chemoenzymatic method for glycomics: Isolation, identification, and quantitation.

Shuang Yang1, Abigail Rubin1, Shadi Toghi Eshghi1

  • 1Department of Pathology, Johns Hopkins University, Baltimore, USA.

Proteomics
|September 22, 2015
PubMed
Summary
This summary is machine-generated.

This review covers analytical methods for glycan analysis, including isolation, identification, and quantitation. Advances in derivatization and solid-phase techniques enhance glycan detection and quantification in biological samples.

Keywords:
GlycomicsGlycoproteomicsIsobaric labelingMass spectrometryiGIG

More Related Videos

Identification and Characterization of Protein Glycosylation using Specific Endo- and Exoglycosidases
09:54

Identification and Characterization of Protein Glycosylation using Specific Endo- and Exoglycosidases

Published on: December 26, 2011

37.7K
Glycomics-Guided Glycoproteomics Facilitates Comprehensive Profiling of the Glycoproteome in Complex Tumor Microenvironments
10:59

Glycomics-Guided Glycoproteomics Facilitates Comprehensive Profiling of the Glycoproteome in Complex Tumor Microenvironments

Published on: February 7, 2025

2.0K

Related Experiment Videos

Last Updated: Apr 3, 2026

A Quantitative Glycomics and Proteomics Combined Purification Strategy
11:38

A Quantitative Glycomics and Proteomics Combined Purification Strategy

Published on: March 8, 2016

15.6K
Identification and Characterization of Protein Glycosylation using Specific Endo- and Exoglycosidases
09:54

Identification and Characterization of Protein Glycosylation using Specific Endo- and Exoglycosidases

Published on: December 26, 2011

37.7K
Glycomics-Guided Glycoproteomics Facilitates Comprehensive Profiling of the Glycoproteome in Complex Tumor Microenvironments
10:59

Glycomics-Guided Glycoproteomics Facilitates Comprehensive Profiling of the Glycoproteome in Complex Tumor Microenvironments

Published on: February 7, 2025

2.0K

Area of Science:

  • Analytical Chemistry
  • Biochemistry
  • Glycomics

Background:

  • Glycan analysis is crucial for understanding biological processes.
  • Traditional methods face challenges in sensitivity and throughput.
  • Recent advancements have improved glycan isolation, identification, and quantitation.

Purpose of the Study:

  • To review and discuss current analytical methods for glycan analysis.
  • To highlight progress in glycan derivatization and detection techniques.
  • To explore the advantages of solid-phase approaches in glycomics.

Main Methods:

  • Glycan isolation and extraction techniques.
  • Derivatization methods: permethylation and carbodiimide coupling/esterification.
  • Separation and detection: electrophoresis, chromatography, and mass spectrometry imaging.

Main Results:

  • Derivatization is essential for glycan identification and quantitation.
  • Fluorogenic labeling enables sensitive detection via fluorescent detection.
  • Solid-phase methods, including glycoprotein immobilization and on-tissue mass spectrometry imaging, offer advantages over solution-phase methods.

Conclusions:

  • Significant progress has been made in analytical methods for glycan analysis.
  • Solid-phase techniques and advanced derivatization strategies are key to improved glycan quantification.
  • This review provides a comprehensive overview of current methodologies in glycomics research.