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Updated: Apr 3, 2026

Isolation of Cortical Microglia with Preserved Immunophenotype and Functionality From Murine Neonates

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Published on: January 30, 2014

[CEREBRAL MICROGLIA AND MICROGLIAL MARKERS].

D E Korzhevskiy, O V Kirik

Morfologiia (Saint Petersburg, Russia)

|September 23, 2015

Summary

This study systematizes immunocytochemical methods for identifying microglia, focusing on key protein markers like Iba-1 and CD11b. It aids researchers in accurately detecting and understanding these crucial brain cells.

Area of Science:

Neuroscience
Immunology
Cell Biology

Background:

Growing interest in microglial organization and function.
Ambiguity in current immunocytochemical identification methods requires clarification.

Purpose of the Study:

To systematize modern immunocytochemical methods for microglia identification.
To characterize key microglial protein markers and their expression in normal and activated states.

Main Methods:

Review and analysis of immunocytochemical labeling techniques.
Focus on protein markers such as Iba-1, CD11b, CD68, and HLA-DR.
Integration of data on microglial origin and structure.

Main Results:

Detailed characterization of commonly used microglial markers.

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Last Updated: Apr 3, 2026

Isolation of Cortical Microglia with Preserved Immunophenotype and Functionality From Murine Neonates

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Published on: January 30, 2014

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Explanation of marker expression in both resting and activated microglia.

Systematized approach to immunocytochemical identification.

Conclusions:

Provides a clear guide for microglia identification using immunocytochemistry.
Highlights the importance of specific protein markers for accurate cell characterization.
Contributes to a better understanding of microglial roles in health and disease.