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Related Experiment Video

Updated: Jan 1, 2026

Robust and Highly Reproducible Generation of Cortical Brain Organoids for Modelling Brain Neuronal Senescence In Vitro
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Modeling mouse and human development using organoid cultures.

Meritxell Huch1, Bon-Kyoung Koo2

  • 1Wellcome Trust/Cancer Research UK Gurdon Institute, Henry Wellcome Building of Cancer and Developmental Biology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QN, UK Wellcome Trust - Medical Research Council Stem Cell Institute, University of Cambridge, Gleeson Building, Tennis Court Road, Cambridge CB2 1QR, UK Department of Physiology, Development and Neuroscience, University of Cambridge, Cambridge CB2 3DY, UK m.huch@gurdon.cam.ac.uk bkk25@cam.ac.uk.

Development (Cambridge, England)
|September 24, 2015
PubMed
Summary
This summary is machine-generated.

Three-dimensional (3D) cultures enable embryonic stem cells (ESCs) and adult stem cells (AdSCs) to form organoids, mimicking human development. This technology advances disease modeling and regenerative medicine.

Keywords:
3D-Organoid CultureAdult Stem CellDisease ModelingEmbryonic Stem CellGenetic Engineering

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Area of Science:

  • Developmental Biology
  • Stem Cell Biology
  • Regenerative Medicine

Background:

  • Three-dimensional (3D) in vitro cultures are novel systems for studying tissue development and stem cell behavior.
  • Embryonic stem cells (ESCs) and adult stem cells (AdSCs) self-organize into organoids in 3D, mimicking in vivo development.

Purpose of the Study:

  • To review recent advances in generating pluripotent stem cell- and AdSC-derived organoids.
  • To highlight the potential of organoids for understanding human development.
  • To explore applications in disease modeling and regenerative medicine.

Main Methods:

  • Culturing embryonic stem cells (ESCs) in a 3D environment.
  • Culturing tissue-resident adult stem cells (AdSCs) in a 3D environment.
  • Generating and analyzing stem cell-derived organoids.

Main Results:

  • ESC-derived organoids mimic early tissue patterning of endoderm and ectoderm.
  • AdSC-derived organoids can be propagated in vitro long-term.
  • Organoid formation recapitulates aspects of in vivo development and tissue organization.

Conclusions:

  • 3D organoid cultures provide powerful models for studying human development ex vivo.
  • Organoids derived from ESCs and AdSCs are promising for disease modeling and personalized regenerative medicine.
  • This technology facilitates gene repair strategies for therapeutic applications.