Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Individual and Sequential Chromatin Immunoprecipitation Protocols.

Mayra Furlan-Magaril1,2, Félix Recillas-Targa3

  • 1Nuclear Dynamics Programme, The Babraham Institute, Cambridge, CB22 3AT, UK.

Methods in Molecular Biology (Clifton, N.J.)
|September 26, 2015
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Metabolic disruptions through a three-dimensional genomic lens.

Current opinion in structural biology·2026
Same author

Structural variants disrupt chromatin architecture.

Nature reviews. Cancer·2026
Same author

NR5A2 controls gene expression and chromatin contacts of essential circadian metabolic genes in the liver.

Cellular and molecular life sciences : CMLS·2026
Same author

3D Chromatin Architecture During Early Development: New Methods and New Findings.

Methods in molecular biology (Clifton, N.J.)·2026
Same author

Effects of topological domain disruption on transcriptional regulation are chromatin context dependent.

Epigenetics & chromatin·2026
Same author

The Genomic Kaleidoscope: On the Hidden Dimensions of Within-Species Genomic Diversity.

Genome biology and evolution·2025
Same journal

Tracking Synthetic Adhesins on Bacterial Surfaces with Immunofluorescence Microscopy.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Post-Selection Methods for Analyzing mRNA Display Selections and Optimization of Hits.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

High-Performance Computing in Tandem Mass Spectrometry (MS/MS) Peptide Identification.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Engineering and Adapting Disulfide-Containing Proteins to Enable Intracellular Functionality.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

AI-Driven Protein Research: From Prediction to Design.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Methods for the In Vitro Selection of Protein and Peptide Libraries Using mRNA Display.

Methods in molecular biology (Clifton, N.J.)·2026
See all related articles

Determining protein co-localization on chromatin is crucial for understanding gene regulation. The ChIP-re-ChIP assay directly assesses in vivo protein interactions at specific genomic locations.

Area of Science:

  • Molecular Biology
  • Genetics
  • Epigenetics

Background:

  • Gene expression is regulated by transcription factors and cofactors interacting at DNA regulatory elements.
  • Understanding protein co-localization on chromatin is essential for deciphering gene regulation mechanisms.
  • Chromatin immunoprecipitation (ChIP) is widely used but cannot confirm simultaneous protein presence at a single genomic site.

Purpose of the Study:

  • To introduce and validate the ChIP-re-ChIP assay for direct assessment of in vivo protein co-localization.
  • To provide a method for determining simultaneous occupancy of proteins or histone modifications at specific genomic locations.

Main Methods:

  • Development and application of the ChIP-re-ChIP assay.
  • Utilizing double and independent rounds of immunoprecipitation.
Keywords:
ChromatinGATA-1Histone modificationsImmunoprecipitationProtein-DNA interactionTelomeric position effectYY-1

Related Experiment Videos

  • Employing high-quality ChIP-grade antibodies for protein or histone modification detection.

    • Main Results:

    • The ChIP-re-ChIP assay enables direct determination of in vivo protein co-localization on chromatin.
    • This method confirms the simultaneous presence of multiple proteins or histone modifications at the same genomic locus.
    • Successful application of ChIP-re-ChIP provides insights into protein interactions within the chromatin fiber.

    Conclusions:

    • The ChIP-re-ChIP assay is a valuable tool for studying the spatial organization of regulatory proteins in vivo.
    • This technique enhances our understanding of the functional interplay of proteins at specific genomic sites.
    • ChIP-re-ChIP advances the study of gene regulation by resolving simultaneous protein occupancy.