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Reusable Single Cell for Iterative Epigenomic Analyses

Reusable Single Cell for Iterative Epigenomic Analyses

Published on: February 11, 2022

Research highlights: microfluidic-enabled single-cell epigenetics.

Manjima Dhar¹, Reem Khojah, Andy Tay

¹Department of Bioengineering, University of California, Los Angeles, CA 90095, USA. dicarlo@ucla.edu.

|September 26, 2015

Summary

This summary is machine-generated.

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Understanding single-cell epigenetic heterogeneity is crucial for biology and disease. New microfluidic techniques enable detailed analysis of mRNA and chromatin accessibility in individual cells, advancing our knowledge.

Area of Science:

Cellular biology
Epigenetics
Genomics

Background:

Individual cells exhibit functional diversity, with epigenetic variations driving differences in gene expression across tissues.
Epigenetic heterogeneity is vital for normal tissue development and implicated in diseases like cancer.
Bulk analysis methods obscure cell-to-cell variations, limiting insights into biological processes and disease states.

Purpose of the Study:

To highlight recent advancements in single-cell analysis techniques.
To emphasize the importance of understanding epigenetic heterogeneity at the single-cell level.
To discuss the role of microfluidics in enabling these new technologies.

Main Methods:

Analysis of mRNA profiles from thousands of single cells.

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Last Updated: Apr 3, 2026

Reusable Single Cell for Iterative Epigenomic Analyses

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A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

Published on: October 15, 2013

An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing

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Published on: May 23, 2018

Assessment of chromatin accessibility and histone marks in single to hundreds of cells.

Application of microfluidics and microfabrication technologies for single-cell epigenetic analysis.

Main Results:

Recent techniques allow for the profiling of mRNA in thousands of single cells.
Methods are emerging to analyze chromatin accessibility and histone marks in small cell populations.
Microfluidics and microfabrication are key enablers of these single-cell analyses.

Conclusions:

Single-cell epigenetic analysis is rapidly advancing.
These new techniques are essential for a deeper understanding of biological complexity and disease.
Microfluidics will continue to drive innovation in routine single-cell epigenetic studies.