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[Study on Optimum Extraction Process of Water Soluble Protein from Coicis Semen]
Objective:
To optimize the extraction process of water soluble protein from Coicis Semen.
Methods:
Coicis Semen was used to extract protein with cysteine hydrochloride aqueous solution in alkaline environment. The single factor test was conducted with the factors of extraction temperature, extraction pH, solid-liquid ratio and extraction time. In combination with single factor test results, a 4 factors 3 levels orthogonal experiment was designed with the factors of extraction temperature, extraction pH, solid-liquid ratio and extraction time. The effect of various factors on the results and the interactions were analyzed by range analysis, variance analysis and partial least squares regression analysis.
Results:
The optimum extraction process of water soluble protein from Coicis Semen was as follows: the extraction temperature was 40 °C , the extraction pH was 10, the solid-liquid ratio was 1:20 and the extraction time was 4 h. The interaction of temperature and solid-liquid ratio, as well as temperature and extraction time would lower protein extraction rate.
Conclusion:
The optimized extraction process is economical, simple, reasonable and practicable.
