Related Experiment Video

Updated: Apr 1, 2026

In Vitro Selection of Engineered Transcriptional Repressors for Targeted Epigenetic Silencing
10:44

In Vitro Selection of Engineered Transcriptional Repressors for Targeted Epigenetic Silencing

Published on: May 5, 2023

2.2K

Functional Domains of ZFP809 Essential for Nuclear Localization and Gene Silencing

Yu Ichida1, Yuko Utsunomiya1, Toru Yasuda1

  • 1Department of Human Genetics, National Center for Child Health and Development, 2-10-1 Okura, Setagaya, Tokyo, 157-8535, Japan.

Plos One
|September 30, 2015
PubMed

Insights

Zinc finger protein 809 (ZFP809) silences transduced genes by binding retroviral vectors. The KRAB A box is essential for this gene silencing, with zinc fingers playing accessory roles.

Area of Science:

  • Molecular Biology
  • Epigenetics
  • Gene Regulation

Background:

  • Zinc finger protein 809 (ZFP809), a KRAB-ZFP family member, is highly expressed in mouse immature cells.
  • ZFP809 inhibits transduced gene expression from MoMLV-type retroviral vectors via epigenetic silencing.
  • The specific domains of ZFP809 responsible for gene silencing were previously undetermined.

Purpose of the Study:

  • To identify the critical domains of ZFP809 responsible for gene silencing.
  • To investigate the roles of ZFP809's KRAB domain and zinc fingers in gene silencing.
  • To understand the interaction between ZFP809, KAP1, and retroviral vectors.

Main Methods:

  • Generated and analyzed truncated and mutated ZFP809 proteins.
  • Assessed subcellular localization, gene silencing ability, and PBS binding affinity.
  • Investigated the interaction between ZFP809 and KAP1.

Main Results:

  • The KRAB A box of ZFP809 is essential for gene silencing, subcellular localization, and PBS binding.
  • Specific zinc fingers contribute accessory roles in ZFP809-mediated gene silencing.
  • Interaction between KAP1 and the ZFP809 KRAB A box is crucial for KAP1-dependent gene silencing.

Conclusions:

  • The KRAB A box is the primary functional domain for ZFP809-mediated gene silencing.
  • Zinc fingers modulate ZFP809's gene silencing activity.
  • KAP1 interaction with the KRAB A box is vital for ZFP809's epigenetic control of target genes.

More Related Videos

HOX Loci Focused CRISPR/sgRNA Library Screening Identifying Critical CTCF Boundaries10:10

HOX Loci Focused CRISPR/sgRNA Library Screening Identifying Critical CTCF Boundaries

Published on: March 31, 2019

8.9K
Lentiviral Vector Platform for the Efficient Delivery of Epigenome-editing Tools into Human Induced Pluripotent Stem Cell-derived Disease Models13:47

Lentiviral Vector Platform for the Efficient Delivery of Epigenome-editing Tools into Human Induced Pluripotent Stem Cell-derived Disease Models

Published on: March 29, 2019

10.5K

Related Concept Videos

Conservation of Protein Domains Over Different Proteins02:26

Conservation of Protein Domains Over Different Proteins

Protein domains are small structurally independent units that are part of a single amino acid chain.  Although these domains are often structurally independent, they may rely on synergistic effects to perform their functions as part of a larger protein. Protein domains may be conserved within the same organism, as well as across different organisms.
A limited set of protein domains often duplicate and recombine during evolution. These domains can be organized in different combinations to...
15.0K
Nuclear Localization Signals and Import01:46

Nuclear Localization Signals and Import

Proteins targeted to the nucleus carry short stretches of amino acid sequences called the nuclear localization signal or NLS. Classical nuclear localization signals are of two types: monopartite and bipartite NLS. Monopartite classical NLS (cNLS) consists of a single cluster of 4-8 amino acids. Bipartite cNLS consists of two clusters of  2-3 amino acids and a 9-12 residue long proline-rich linker bridging the two clusters. Signal clusters are rich in positively charged amino acids such as...
8.3K
Regulation of Nuclear Protein Sorting01:45

Regulation of Nuclear Protein Sorting

Nuclear protein sorting regulates nucleus composition and gene expression, crucial for determining the fate of a eukaryotic cell. Hence, the entry and exit of molecules across the nuclear envelope is a tightly controlled process. Nuclear protein sorting can be inhibited by one of the following ways: 1) masking cargo signal sequences, 2) modifying the nuclear receptor's affinity for cargo, 3) controlling the nuclear pore size, 4) retaining the cargo during its transit to the cytosol or the...
3.5K
Nuclear Export of mRNA02:31

Nuclear Export of mRNA

5.6K
Nuclear Export of mRNA02:31

Nuclear Export of mRNA

Before mRNAs are exported to the cytoplasm, it is crucial to check each mRNA for structural and functional integrity. Eukaryotic cells use several different mechanisms, collectively known as mRNA surveillance, to look for irregularities in mRNAs. Irregular or aberrant mRNA are rapidly degraded by various enzymes. If a defective mRNA escapes the surveillance, it would be translated into a protein which would either be non-functional or not function properly. One of the primary irregularities in...
9.2K
Chromatin Position Affects Gene Expression02:35

Chromatin Position Affects Gene Expression

Chromatin is the massive complex of DNA and proteins packaged inside the nucleus. The complexity of chromatin folding and how it is packaged inside the nucleus greatly influences  access to genetic information. Generally, the nucleus' periphery is considered transcriptionally repressive, while the cell's interior is considered a transcriptionally active area. 
Topologically Associated Domains (TADs)
The 3-dimensional positioning of chromatin in the nucleus influences the...
25.2K