Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Mitigating and addressing complications in equine laparoscopy.

Journal of the American Veterinary Medical Association·2026

Same author

Uncertainty quantification-based DMEFNet for reliable modelling of heart sound signals.

Scientific reports·2026

Same author

Angiotensin II Promotes the Phenotypic Transition of c-Kit<sup>+</sup> Cardiac Interstitial Cells to Myofibroblasts Through Collagen Receptor Discoidin Domain Receptor 2.

BioFactors (Oxford, England)·2026

Same author

Cl-amidine attenuates neutrophil extracellular trap-enclosed extracellular vesicle (NET-EV)-mediated thrombosis in diabetic mice.

Thrombosis research·2026

Same author

Controlled release of bone morphogenetic protein-2 improves motor function after traumatic brain injury in a rat model.

Journal of materials chemistry. B·2026

Same author

A pilot study to assess feasibility of a computer-assisted surgery system for navigation in the equine cervical spine.

American journal of veterinary research·2026

Same journal

Industry updates in advanced therapy medicinal products and regenerative medicine - May 2026.

Regenerative medicine·2026

Same journal

Ethical, legal, and social issues associated with human fetal tissue research in Japan.

Regenerative medicine·2026

Same journal

The future of hematopoietic stem cell and stem cell gene therapy for metabolic diseases.

Regenerative medicine·2026

Same journal

Industry updates in advanced therapy medicinal products and regenerative medicine - April 2026.

Regenerative medicine·2026

Same journal

Optimizing strategies in tendon tissue engineering through effective scaffold design: overview of recent advancements.

Regenerative medicine·2026

Same journal

Establishment and qualification of a GMP-compliant induced pluripotent stem cell master cell bank for clinical applications under the Korean MFDS framework.

Regenerative medicine·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Apr 1, 2026

Treatment of Osteochondral Defects in the Rabbit's Knee Joint by Implantation of Allogeneic Mesenchymal Stem Cells in Fibrin Clots

Treatment of Osteochondral Defects in the Rabbit's Knee Joint by Implantation of Allogeneic Mesenchymal Stem Cells in Fibrin Clots

Published on: May 21, 2013

Microsphere-based gradient implants for osteochondral regeneration: a long-term study in sheep.

Neethu Mohan^1,2, Vineet Gupta³, Banu Priya Sridharan³

¹Department of Chemical & Petroleum Engineering, University of Kansas, Lawrence, KS 66045, USA.

Regenerative Medicine

|September 30, 2015

Summary

This summary is machine-generated.

Gradient scaffolds show promise for cartilage repair, outperforming microfracture in a sheep study. This advanced osteochondral approach regenerates hyaline-like cartilage with superior mechanical properties.

Keywords:

cartilage regeneration chondroitin sulfate gradients of signals microfracture microsphere-based scaffold osteochondral tissue engineering

More Related Videos

Matrix-assisted Autologous Chondrocyte Transplantation for Remodeling and Repair of Chondral Defects in a Rabbit Model

Matrix-assisted Autologous Chondrocyte Transplantation for Remodeling and Repair of Chondral Defects in a Rabbit Model

Published on: May 21, 2013

Implantation of Ferumoxides Labeled Human Mesenchymal Stem Cells in Cartilage Defects

Implantation of Ferumoxides Labeled Human Mesenchymal Stem Cells in Cartilage Defects

Published on: April 5, 2010

Related Experiment Videos

Last Updated: Apr 1, 2026

Treatment of Osteochondral Defects in the Rabbit's Knee Joint by Implantation of Allogeneic Mesenchymal Stem Cells in Fibrin Clots

Treatment of Osteochondral Defects in the Rabbit's Knee Joint by Implantation of Allogeneic Mesenchymal Stem Cells in Fibrin Clots

Published on: May 21, 2013

Matrix-assisted Autologous Chondrocyte Transplantation for Remodeling and Repair of Chondral Defects in a Rabbit Model

Matrix-assisted Autologous Chondrocyte Transplantation for Remodeling and Repair of Chondral Defects in a Rabbit Model

Published on: May 21, 2013

Implantation of Ferumoxides Labeled Human Mesenchymal Stem Cells in Cartilage Defects

Implantation of Ferumoxides Labeled Human Mesenchymal Stem Cells in Cartilage Defects

Published on: April 5, 2010

Area of Science:

Biomaterials Science
Orthopedic Surgery
Regenerative Medicine

Background:

Microfracture technique has limitations in regenerating hyaline cartilage.
Need for improved cartilage repair strategies in osteochondral defects.

Purpose of the Study:

Directly compare microfracture with a novel osteochondral approach using microsphere-based gradient plugs.
Evaluate the efficacy of gradient scaffolds for cartilage regeneration.

Main Methods:

Developed PLGA-based scaffolds with opposing gradients of chondroitin sulfate and β-tricalcium phosphate.
Conducted a 1-year repair study in a large animal model (sheep).

Main Results:

Microfracture repair resulted in fibrous tissue with fissures and degeneration.
Gradient plug repair regenerated cartilage with mechanical properties equal to or superior to hyaline cartilage.
Regenerated cartilage exhibited lacunated cells and a stable matrix characteristic of hyaline cartilage.

Conclusions:

Gradient scaffolds represent a novel approach for osteochondral defect repair.
This study is the first to report on gradient scaffolds in a long-term, large animal model.
The findings suggest gradient scaffolds have the potential for superior cartilage repair compared to microfracture.