A Standardized Method of Isolating Adipose-Derived Stem Cells for Clinical Applications
Edoardo Raposio1, Giorgia Caruana, Maira Petrella
1From the *Department of Surgical Sciences, Plastic Surgery Division, University of Parma; Cutaneous, Mininvasive, Regenerative and Plastic Surgery Unit; and †Division of Hematology, Department of Clinical and Experimental Medicine, Parma University Hospital, Parma, Italy.
Abstract:
White adipose tissue is the most abundant and accessible source of stem cells in the adult human body. In this paper, we present a standardised and safe method of isolating and maximizing the number of adipose-derived stem cells (ASCs) from conventional liposuction for clinical applications, which was carried out through both mechanical (centrifuge) and enzymatic (collagenase) means. Isolated cells were characterized through flow cytometry assay. Gathered data showed a greater amount (9.06 × 10(5) ASCs from 100 mL of adipose tissue) of isolated ASCs compared to previous protocol, also with high (99%) cell vitality; the procedure we presented is easy and fast (80 minutes), allowing collecting a significative number of mesenchymal stem cells, which can be used for clinical purposes, such as wound healing.
