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Updated: Apr 1, 2026
Luminescence Lifetime Imaging of O2 with a Frequency-Domain-Based Camera System
Published on: December 16, 2019
Multi-Level, Multi Time-Scale Fluorescence Intermittency of Photosynthetic LH2 Complexes: A Precursor of
Mario Schörner1, Sebastian Reinhardt Beyer1, June Southall2
1Experimental Physics IV and Bayreuth Institute for Macromolecular Research (BIMF), University of Bayreuth , 95447 Bayreuth, Germany.
Abstract:
The light harvesting complex LH2 is a chromoprotein that is an ideal system for studying protein dynamics via the spectral fluctuations of the emission of its intrinsic chromophores. We have immobilized these complexes in a polymer film and studied the fluctuations of the fluorescence intensity from individual complexes over 9 orders of magnitude in time. Combining time-tagged detection of single photons with a change-point analysis has allowed the unambigeous identification of the various intensity levels due to the huge statistical basis of the data set. We propose that the observed intensity level fluctuations reflect conformational changes of the protein backbone that might be a precursor of the mechanism from which nonphotochemical quenching of higher plants has evolved.
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