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Analyzing the Effect of Aging on CD8+ T-Cell Phenotype Using Flow Cytometry

Min Sun Shin1, Insoo Kang2

  • 1Section of Rheumatology, Department of Internal Medicine, Yale School of Medicine, 300 Cedar Street, New Haven, CT, 06520, USA.

Methods in Molecular Biology (Clifton, N.J.)
|October 1, 2015
PubMed

One of the most noticeable changes in T-cell immunity with aging is the expansion of memory CD8+ T cells, with a decline in naïve phenotype T cells that reflects both diminished thymopoiesis and the effects of chronic antigenic stimulation with age. Flow cytometry is a useful tool in evaluating immune cells including the phenotype characteristics of different T-cell subsets. Here, we show flow cytometric methods measuring the different subsets of human CD8+ T cells that change with aging.

Keywords:
Flow cytometryHumanT cells

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