Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Fluorescence and Phosphorescence: Instrumentation01:25

Fluorescence and Phosphorescence: Instrumentation

1.9K
Fluorometers and spectrofluorometers are two types of instruments used for measuring molecular fluorescence. These instruments differ in how they select excitation and emission wavelengths and the type of light sources they utilize. Fluorometers use absorption interference filters to choose excitation and emission wavelengths. The excitation source in a fluorometer is typically a low-pressure mercury vapor lamp that emits intense lines distributed throughout the ultraviolet and visible regions.
1.9K
Photoluminescence: Applications01:14

Photoluminescence: Applications

1.2K
Photoluminescence offers a wide range of applications due to its inherent sensitivity and selectivity. This technique allows for both direct and indirect analyses of the analyte. Direct quantitative analysis is possible when the analyte exhibits a favorable quantum yield for fluorescence or phosphorescence. However, an indirect analysis may be feasible if the analyte is not fluorescent or phosphorescent, or if the quantum yield is unfavorable. Indirect methods include reacting the analyte with...
1.2K
Flame Photometry: Overview01:02

Flame Photometry: Overview

1.9K
Flame photometry, also known as flame emission spectrometry, is a technique used for the qualitative and quantitative analysis of elements present in a sample using a flame as the source of excitation energy. The concept of flame photometry was realized in the early 1860s by Kirchhoff and Bunsen, who discovered that specific elements emit characteristic radiation when excited in flames. The first instrument developed for this purpose was used to measure sodium (Na) in plant ash using a Bunsen...
1.9K
Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

13.7K
Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
13.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

QTL mapping for flowering time in a maize-teosinte population under well-watered and water-stressed conditions.

Molecular breeding : new strategies in plant improvement·2023
Same author

Reconstitution of phytochrome A-mediated light modulation of the ABA signaling pathways in yeast.

Proceedings of the National Academy of Sciences of the United States of America·2023
Same author

Response characteristics of MAPbBr<sub>3</sub> direct conversion X-ray detectors based on measurements and Monte Carlo simulation.

Heliyon·2023
Same author

Expression Profiling Reveals the Possible Involvement of the Ubiquitin-Proteasome Pathway in Abiotic Stress Regulation in <i>Gracilariopsis lemaneiformis</i>.

International journal of molecular sciences·2023
Same author

Natural polymorphisms in ZmIRX15A affect water-use efficiency by modulating stomatal density in maize.

Plant biotechnology journal·2023
Same author

Association between vaginal microbiomes and neonatal septicemia in pregnant women with preterm premature rupture of membranes based on metagenome sequencing.

American journal of translational research·2023

Related Experiment Video

Updated: Apr 1, 2026

High-resolution Thermal Micro-imaging Using Europium Chelate Luminescent Coatings
09:01

High-resolution Thermal Micro-imaging Using Europium Chelate Luminescent Coatings

Published on: April 16, 2017

8.2K

Fluorescence intensity ratio method for temperature sensing.

Yuan Zhou, Feng Qin, Yangdong Zheng

    Optics Letters
    |October 1, 2015
    PubMed
    Summary

    A new thermometry method uses the ratio of fluorescence peak intensities in Eu3+-doped CaWO4. This method accurately measures temperature by observing spectral line broadening, offering a sensitive approach for thermal monitoring.

    More Related Videos

    Highly Sensitive and Rapid Fluorescence Detection with a Portable FRET Analyzer
    08:27

    Highly Sensitive and Rapid Fluorescence Detection with a Portable FRET Analyzer

    Published on: October 1, 2016

    9.6K
    Near-Infrared Temperature Measurement Technique for Water Surrounding an Induction-heated Small Magnetic Sphere
    08:52

    Near-Infrared Temperature Measurement Technique for Water Surrounding an Induction-heated Small Magnetic Sphere

    Published on: April 30, 2018

    8.8K

    Related Experiment Videos

    Last Updated: Apr 1, 2026

    High-resolution Thermal Micro-imaging Using Europium Chelate Luminescent Coatings
    09:01

    High-resolution Thermal Micro-imaging Using Europium Chelate Luminescent Coatings

    Published on: April 16, 2017

    8.2K
    Highly Sensitive and Rapid Fluorescence Detection with a Portable FRET Analyzer
    08:27

    Highly Sensitive and Rapid Fluorescence Detection with a Portable FRET Analyzer

    Published on: October 1, 2016

    9.6K
    Near-Infrared Temperature Measurement Technique for Water Surrounding an Induction-heated Small Magnetic Sphere
    08:52

    Near-Infrared Temperature Measurement Technique for Water Surrounding an Induction-heated Small Magnetic Sphere

    Published on: April 30, 2018

    8.8K

    Area of Science:

    • Materials Science
    • Spectroscopy
    • Thermometry

    Background:

    • Accurate temperature measurement is crucial in various scientific and industrial applications.
    • Fluorescence-based thermometry offers non-contact and potentially high-resolution temperature sensing.
    • Europium (Eu3+)-doped materials are known for their distinct luminescent properties.

    Purpose of the Study:

    • To develop and validate a novel thermometry method using fluorescence spectroscopy.
    • To investigate the temperature-dependent spectral changes in Eu3+-doped CaWO4.
    • To quantify the sensitivity of the proposed thermometry technique.

    Main Methods:

    • Utilized a 405 nm laser for excitation of Eu3+-doped CaWO4.
    • Developed a thermometry approach based on the valley-to-peak ratio (VPR) of fluorescence emissions.
    • Analyzed spectral changes, specifically the broadening of 5D0 to 7F2 Stark sublevels, with varying temperature.

    Main Results:

    • Observed a monotonic increase in the VPR with increasing temperature from 303 K to 573 K.
    • Spectrum analysis confirmed homogeneous broadening of spectral lines as the primary cause for the observed VPR change.
    • Achieved a relative sensitivity (S(r)) on the order of 10(-4) K(-1) within the experimental range.

    Conclusions:

    • The VPR method provides a reliable approach for thermometry in Eu3+-doped CaWO4.
    • Homogeneous spectral broadening is a key factor enabling temperature sensing via fluorescence.
    • The developed method demonstrates good sensitivity for practical thermometry applications.