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Updated: Apr 1, 2026

An Integrated System to Remotely Trigger Intracellular Signal Transduction by Upconversion Nanoparticle-mediated Kinase Photoactivation

An Integrated System to Remotely Trigger Intracellular Signal Transduction by Upconversion Nanoparticle-mediated Kinase Photoactivation

Published on: August 30, 2017

A NIR-remote controlled upconverting nanoparticle: an improved tool for living cell dye-labeling.

Bin Zheng¹, Xiaoqun Gong, Hanjie Wang

¹School of Life Sciences, Tianjin University, Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), 92 Weijin Road, Nankai District, Tianjin 300072, People's Republic of China.

|October 1, 2015

Summary

Researchers developed novel core-shell nanocarriers to improve organic dye delivery for cell imaging. These nanocarriers enhance dye uptake and enable near-infrared light-triggered release, overcoming limitations of free dyes in complex cellular environments.

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Area of Science:

Biotechnology
Nanotechnology
Cellular Imaging

Background:

Organic dye uptake and stability in living cells are limited by cellular environments, affecting fluorescent imaging.
Developing efficient and controllable dye delivery systems is crucial for advanced cell imaging applications.

Purpose of the Study:

To design and synthesize core-shell nanocarriers for enhanced organic dye delivery and controlled release in living cells.
To improve cellular uptake efficiency and imaging stability compared to free organic dyes.
To achieve spatiotemporal control over dye release using near-infrared (NIR) light.

Main Methods:

Fabrication of upconverting nanocrystal (UCN) core-shell nanocarriers coated with mesoporous silica (mSiO2), folate (FA), and azobenzene (Azo).
Loading of organic dyes (rhodamine, DAPI) into the mesoporous silica shell.
Utilizing UCNs to convert NIR light into UV/visible light, activating azobenzene for dye release.
Evaluating cellular uptake efficiency and dye release kinetics under NIR irradiation.

Main Results:

Successfully synthesized UCNs@mSiO2-(FA and Azo) core-shell nanocarriers.
Demonstrated significantly improved cellular uptake of model dyes (rhodamine, DAPI) compared to free dyes.
Achieved NIR light-triggered, dose-dependent release of organic dyes, enabling spatiotemporal control.
Showcased enhanced stability of fluorescent imaging effects using nanocarriers.

Conclusions:

The developed nanocarriers offer a promising platform for precise control of dye delivery in living cell imaging.
This approach enhances imaging quality and opens new possibilities for studying cellular dynamics and diagnostics.
NIR-triggered dye release provides a non-invasive method for spatiotemporal control in biotechnological applications.