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Updated: Apr 1, 2026

Measuring the Effects of Bacteria and Chemicals on the Intestinal Permeability of Caenorhabditis elegans
Published on: December 3, 2019
DIARRHEA OUTBREAK IN PERNAMBUCO, BRAZIL, ASSOCIATED WITH A HEAT-STABLE CYTOTOXIC ENTEROTOXIN PRODUCED BY Aeromonas
Ana Carolina Amaral Lopes1, Luciano Moura Martins2, Maria Silvia Viccari Gatti1
1Evolution and Bioagents Department, State University of Campinas, Campinas, SP, BR.
Abstract:
In the present study enterotoxic and cytotoxic activities of twenty Aeromonas caviae strains were examined. They originated from fecal specimens of patients with acute diarrhea during an outbreak in Brazil in 2004. Culture supernatants of fourteen strains (70%) caused fluid accumulation in rabbit ileal intestinal loops and in suckling mice assays, and also showed a cytotoxic activity in Vero and Caco-2 cells. The enterotoxic and cytotoxic factors were heat-stable after culture supernatants treatment at 100 ºC. The results revealed that A. caviae strains produce a putative diarrheagenic virulence factor, a heat-stable cytotoxic enterotoxin that could be linked to the diarrhea outbreak that took place in Brazil.
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