Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

MicroRNAs01:22

MicroRNAs

4.3K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
4.3K
MicroRNAs01:22

MicroRNAs

24.7K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
24.7K
MicroRNAs01:22

MicroRNAs

12.0K
12.0K
RNA Interference01:23

RNA Interference

28.6K
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
28.6K
RNA Interference01:23

RNA Interference

7.8K
7.8K
Experimental RNAi02:15

Experimental RNAi

8.3K
RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
8.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Bacteria are a major determinant of Orsay virus transmission and infection in <i>Caenorhabditis elegans</i>.

eLife·2024
Same author

Genetic variants that modify neuroendocrine gene expression and foraging behavior of <i>C. elegans</i>.

Science advances·2024
Same author

Genetic Variants That Modify the Neuroendocrine Regulation of Foraging Behavior in <i>C. elegans</i>.

bioRxiv : the preprint server for biology·2023
Same author

Bacteria Are a Major Determinant of Orsay Virus Transmission and Infection in <i>Caenorhabditis elegans</i>.

bioRxiv : the preprint server for biology·2023
Same author

Asymmetric inheritance of RNA toxicity in <i>C. elegans</i> expressing CTG repeats.

iScience·2022
Same author

Neuronal control of maternal provisioning in response to social cues.

Science advances·2021

Related Experiment Video

Updated: Apr 1, 2026

CRISPR Gene Editing Tool for MicroRNA Cluster Network Analysis
10:40

CRISPR Gene Editing Tool for MicroRNA Cluster Network Analysis

Published on: April 25, 2022

3.0K

RNA Interference and MicroRNA-Mediated Silencing.

Sylvia E J Fischer1

  • 1Departments of Genetics and Molecular Biology, Massachusetts General Hospital, Boston, Massachusetts.

Current Protocols in Molecular Biology
|October 2, 2015
PubMed
Summary
This summary is machine-generated.

RNA interference (RNAi) and microRNA silencing are key gene regulation mechanisms. This overview explains their discovery, function, and application in gene studies and screening.

Keywords:
RNAimicroRNAsilencing

More Related Videos

Biotin-based Pulldown Assay to Validate mRNA Targets of Cellular miRNAs
11:00

Biotin-based Pulldown Assay to Validate mRNA Targets of Cellular miRNAs

Published on: June 12, 2018

14.7K
Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library
08:40

Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library

Published on: April 6, 2012

18.1K

Related Experiment Videos

Last Updated: Apr 1, 2026

CRISPR Gene Editing Tool for MicroRNA Cluster Network Analysis
10:40

CRISPR Gene Editing Tool for MicroRNA Cluster Network Analysis

Published on: April 25, 2022

3.0K
Biotin-based Pulldown Assay to Validate mRNA Targets of Cellular miRNAs
11:00

Biotin-based Pulldown Assay to Validate mRNA Targets of Cellular miRNAs

Published on: June 12, 2018

14.7K
Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library
08:40

Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library

Published on: April 6, 2012

18.1K

Area of Science:

  • Molecular Biology
  • Genetics
  • Gene Regulation

Background:

  • RNA interference (RNAi) and microRNA (miRNA) pathways mediate sequence-specific gene silencing.
  • These small RNA pathways play crucial roles in regulating gene expression.

Purpose of the Study:

  • To provide a comprehensive overview of RNAi and miRNA-mediated gene silencing.
  • To describe the discovery, mechanisms, and functions of small RNAs.
  • To highlight the applications of RNAi in gene function studies and high-throughput screening.

Main Methods:

  • Review of seminal research and current literature on RNAi and miRNA pathways.
  • Description of the molecular mechanisms of small RNA-guided target recognition and silencing.
  • Discussion of experimental approaches for studying small RNA function and applications in model systems.

Main Results:

  • Small RNAs, including miRNAs, are fundamental regulators of gene expression.
  • RNAi technology has become an indispensable tool for functional genomics and target validation.
  • Analysis of endogenous small RNAs provides insights into biological processes and disease.

Conclusions:

  • RNAi and miRNA pathways represent conserved and critical mechanisms for gene regulation.
  • Understanding these pathways is essential for advancing molecular biology and developing novel therapeutic strategies.
  • The application of RNAi in research continues to expand, facilitating high-throughput screening and functional analysis across diverse model systems.