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Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Related Experiment Video

Updated: Apr 1, 2026

A Flow Cytometry-based Assay to Identify Compounds That Disrupt Binding of Fluorescently-labeled CXC Chemokine Ligand 12 to CXC Chemokine Receptor 4

A Flow Cytometry-based Assay to Identify Compounds That Disrupt Binding of Fluorescently-labeled CXC Chemokine Ligand 12 to CXC Chemokine Receptor 4

Published on: March 10, 2018

Measuring Cell-Cell Binding Using Flow-Cytometry.

Zachary C Ruhe¹, Christopher S Hayes^1,2, David A Low^3,4

¹Department of Molecular, Cellular and Developmental Biology, University of California, Santa Barbara, CA, 93106, USA.

Methods in Molecular Biology (Clifton, N.J.)

|October 3, 2015

Summary

This summary is machine-generated.

We developed a simple method to quantify microbial cell-cell adhesion using fluorescence labeling and flow cytometry. This technique allows for rapid and easy measurement of interactions between different microbial populations.

Keywords:

CdiA Contact-dependent growth inhibition Flow cytometry Fluorescent proteins Self/nonself recognition cell–cell binding

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Area of Science:

Microbiology
Cell Biology
Biotechnology

Background:

Cell-cell adhesion is crucial for microbial interactions, influencing both competition and cooperation.
Understanding these adhesion dynamics is key to deciphering microbial community behavior.

Purpose of the Study:

To describe a novel method for quantifying cell-cell adhesion events.
To enable rapid and easy measurement of interactions between differentially labeled microbial populations.

Main Methods:

Utilized fluorescence labeling to distinguish between two distinct cell populations.
Employed flow cytometry for high-throughput observation and quantification of cell-cell adhesion events.

Main Results:

Successfully quantified cell-cell adhesion between two differentially labeled populations.
Demonstrated the speed and ease of the described method.

Conclusions:

The described method provides a robust approach to study microbial cell-cell adhesion.
Fluorescence labeling and flow cytometry offer an efficient tool for analyzing microbial interactions.