Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

3.0K
Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
3.0K
X-ray Diffraction of Biological Samples01:10

X-ray Diffraction of Biological Samples

5.2K
X-ray diffraction or XRD is an analytical tool that utilizes X-rays to study ordered structures such as crystalline organic and inorganic samples, polycrystalline materials, proteins, carbohydrates, and drugs.
According to Bragg's law, when X-rays strike the sample positioned on a stage, the rays are  scattered by the electron clouds around the sample atoms. The  X-ray diffraction or scattering is caused by constructive interference of the X-ray waves that reflect off the internal...
5.2K
Transmission Electron Microscopy01:15

Transmission Electron Microscopy

7.9K
In 1931, physicist Ernst Ruska—building on the idea that magnetic fields can direct an electron beam just as lenses can direct a beam of light in an optical microscope—developed the first prototype of the electron microscope. This development led to the development of the field of electron microscopy. In the transmission electron microscope (TEM), electrons are produced by a hot tungsten element and accelerated by a potential difference in an electron gun, which gives them up to 400...
7.9K
Overview of Microscopy Techniques01:22

Overview of Microscopy Techniques

17.7K
The early pioneers of microscopy opened a window into the invisible world of microorganisms. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes that leveraged nonvisible light, such as fluorescence microscopy that uses an ultraviolet light source and electron microscopy that uses short-wavelength electron beams. These advances significantly improved magnification, image resolution, and contrast. By comparison, the...
17.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Design strategy, functional mechanism and antibacterial applications of metal-organic framework-hyaluronic acid composite.

International journal of biological macromolecules·2026
Same author

Integrating the advantages of two single-pixel imaging schemes via holographic projection in ghost-imaging systems.

Optics express·2026
Same author

Corrigendum: Quantitative investigation of the edge enhancement in in-line phase contrast projections and tomosynthesis provided by distributing microbubbles on the interface between two tissues: a phantom study (2017<i>Phys. Med. Biol</i>.<b>62</b>9357).

Physics in medicine and biology·2026
Same author

Dynamic reconstruction of hydrogen-bond networks in N,N-dimethylformamide-water system revealed by 2D correlation Raman spectroscopy.

The Journal of chemical physics·2026
Same author

Contrast mechanism illuminated by polychromatic X-rays and tomographic correction.

Optics letters·2026
Same author

A versatile miniature laser powder bed fusion setup for in situ observations of keyhole and phase transformation dynamics via high-speed synchrotron imaging and diffraction.

The Review of scientific instruments·2026

Related Experiment Video

Updated: Apr 1, 2026

Non-invasive 3D-Visualization with Sub-micron Resolution Using Synchrotron-X-ray-tomography
08:51

Non-invasive 3D-Visualization with Sub-micron Resolution Using Synchrotron-X-ray-tomography

Published on: May 27, 2008

13.7K

Technical Note: Synchrotron-based high-energy x-ray phase sensitive microtomography for biomedical research.

Huiqiang Liu1, Xizeng Wu2, Tiqiao Xiao3

  • 1Japan Synchrotron Radiation Research Institute, SPring-8, 1-1-1 Kouto, Sayo, Hyogo 679-5198, Japan.

Medical Physics
|October 3, 2015
PubMed
Summary

A new method using generalized phase-attenuation duality (PAD) for propagation-based phase-contrast CT (PPCT) significantly improves high-resolution imaging. This advanced PPCT technique offers superior tissue discrimination and characterization compared to existing methods.

More Related Videos

Using Synchrotron Radiation Microtomography to Investigate Multi-scale Three-dimensional Microelectronic Packages
08:46

Using Synchrotron Radiation Microtomography to Investigate Multi-scale Three-dimensional Microelectronic Packages

Published on: April 13, 2016

10.6K
Dynamic Pore-scale Reservoir-condition Imaging of Reaction in Carbonates Using Synchrotron Fast Tomography
10:18

Dynamic Pore-scale Reservoir-condition Imaging of Reaction in Carbonates Using Synchrotron Fast Tomography

Published on: February 21, 2017

8.9K

Related Experiment Videos

Last Updated: Apr 1, 2026

Non-invasive 3D-Visualization with Sub-micron Resolution Using Synchrotron-X-ray-tomography
08:51

Non-invasive 3D-Visualization with Sub-micron Resolution Using Synchrotron-X-ray-tomography

Published on: May 27, 2008

13.7K
Using Synchrotron Radiation Microtomography to Investigate Multi-scale Three-dimensional Microelectronic Packages
08:46

Using Synchrotron Radiation Microtomography to Investigate Multi-scale Three-dimensional Microelectronic Packages

Published on: April 13, 2016

10.6K
Dynamic Pore-scale Reservoir-condition Imaging of Reaction in Carbonates Using Synchrotron Fast Tomography
10:18

Dynamic Pore-scale Reservoir-condition Imaging of Reaction in Carbonates Using Synchrotron Fast Tomography

Published on: February 21, 2017

8.9K

Area of Science:

  • Medical Imaging
  • X-ray Tomography
  • Phase Contrast Imaging

Background:

  • Propagation-based phase-contrast CT (PPCT) leverages phase-contrast technology in X-ray microtomography.
  • Phase retrieval from angular projections enhances image contrast and enables quantitative imaging.

Purpose of the Study:

  • To demonstrate the validity and advantages of a novel high-resolution PPCT technique.
  • Utilizing the generalized phase-attenuation duality (PAD) method for phase retrieval.

Main Methods:

  • Acquired high-resolution angular projection data of a fish head using a 60-keV monochromatic X-ray beam.
  • Reconstructed images using direct projection data, linearized PAD-based phase retrieval, and generalized PAD-based phase retrieval.
  • Compared reconstructed images based on contrast-to-noise ratio and spatial resolution.

Main Results:

  • Validated the PPCT technique employing the generalized PAD method.
  • Demonstrated superior tissue discrimination and characterization compared to direct PPCT and linearized PAD-based PPCT.
  • Generalized PAD-based PPCT showed enhanced performance in image quality metrics.

Conclusions:

  • The novel PPCT technique using generalized PAD shows significant potential for biomedical imaging.
  • Particularly beneficial for applications demanding high spatial resolution and minimal radiation exposure.
  • Offers improved capabilities for detailed tissue analysis in microtomography.