Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Modern Molecular Taxonomy01:29

Modern Molecular Taxonomy

835
Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...
835
Applications of Molecular Taxonomy01:20

Applications of Molecular Taxonomy

655
Molecular taxonomy has revolutionized the understanding and classification of bacteria, providing precise insights into their diversity, evolutionary relationships, and ecological roles. By utilizing molecular techniques such as DNA sequencing and fingerprinting, researchers have made significant strides in various fields related to bacterial studies.Resolving Taxonomic AmbiguitiesMolecular taxonomy has been instrumental in distinguishing closely related bacterial species initially thought to...
655
Methods of Classification and Identification01:28

Methods of Classification and Identification

1.8K
Bacterial identification relies on a diverse array of techniques to classify and understand microorganisms, each tailored to uncover specific characteristics. Traditional morphological approaches, while still valuable, are limited for closely related or structurally simple organisms. Modern methods integrate biochemical, serological, genetic, and advanced molecular tools to achieve greater accuracy.Morphological and Biochemical TechniquesMorphological characteristics, such as cell shape and...
1.8K
Antibiotic Selection00:57

Antibiotic Selection

62.1K
Overview
62.1K
Evolution of Microbial Genome01:08

Evolution of Microbial Genome

47
Microbial genome evolution is a highly dynamic process shaped by continual gene gain and loss across species and strains. This genomic flexibility allows microorganisms to adapt rapidly to environmental pressures and interactions with other organisms. Central to understanding this diversity is the distinction between the core and pan genomes.The core genome comprises the genes shared by all sampled strains of a species, representing essential functions needed for fundamental cellular processes.
47
Genomic DNA in Prokaryotes00:46

Genomic DNA in Prokaryotes

50.6K
The genome of most prokaryotic organisms consists of double-stranded DNA organized into one circular chromosome in a region of cytoplasm called the nucleoid. The chromosome is tightly wound, or supercoiled, for efficient storage. Prokaryotes also contain other circular pieces of DNA called plasmids. These plasmids are smaller than the chromosome and often carry genes that confer adaptive functions, such as antibiotic resistance.
Genomic Diversity in Bacteria
Although bacterial genomes are much...
50.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Maternal and Newborn Group B <i>Streptococcus</i> Serology in the United States and South Africa.

Open forum infectious diseases·2026
Same author

Post-shift recovery sleep duration and its association with daytime sleepiness among night-shift nurses: a repeated-measures observational study.

Frontiers in public health·2026
Same author

A novel compound heterozygous NBAS variant with HLH and multisystem involvement: expanding the clinical spectrum and literature review.

Frontiers in immunology·2026
Same author

First complete genomic sequencing of recent canine rabies viruses in Sierra Leone suggests cross-border transmission of Africa 2H subclade.

Virologica Sinica·2026
Same author

High circular dichroism response predicted by deep learning in chiral metasurfaces.

Applied optics·2026
Same author

IAR025, a novel anti-DLL3 x CD3-bispecific antibody, displays high antitumor efficacy in preclinical models of small-cell lung cancer.

Cancer immunology, immunotherapy : CII·2026
Same journal

Correction to 'scSuperAnnotator: A platform for benchmarking comparison and visualizing automated cellular annotation methods for scRNA-seq data'.

Nucleic acids research·2026
Same journal

Correction to 'Differentiable partition function calculation for RNA'.

Nucleic acids research·2026
Same journal

Deployment of non-canonical splicing in tunicate genomes is mediated by divergent U2AF function and changing m6A modification in U1 and U6 snRNA.

Nucleic acids research·2026
Same journal

Bacillus subtilis DnaB forms multiple protein-protein interactions essential for DNA replication initiation.

Nucleic acids research·2026
Same journal

Multiple forms of protein-protein and DNA binding are exhibited by BrxC from the BREX phage restriction system.

Nucleic acids research·2026
Same journal

Biosynthesis of glycosylated 5-hydroxycytosine in the DNA of diverse viruses.

Nucleic acids research·2026
See all related articles

Related Experiment Video

Updated: Apr 1, 2026

Heuristic Mining of Hierarchical Genotypes and Accessory Genome Loci in Bacterial Populations
08:03

Heuristic Mining of Hierarchical Genotypes and Accessory Genome Loci in Bacterial Populations

Published on: December 7, 2021

2.9K

BacWGSTdb, a database for genotyping and source tracking bacterial pathogens.

Zhi Ruan1, Ye Feng2

  • 1Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, Hangzhou, 310016, China.

Nucleic Acids Research
|October 4, 2015
PubMed
Summary
This summary is machine-generated.

BacWGSTdb is a new database for bacterial whole genome sequencing typing. It helps identify closely related bacterial isolates and their clinical information for tracking pathogens.

More Related Videos

Generating Whole Bacterial Genomes from Clinical Samples using a Target Enrichment Workflow
10:44

Generating Whole Bacterial Genomes from Clinical Samples using a Target Enrichment Workflow

Published on: August 15, 2025

1.3K
Characterization of a Pathogenic Escherichia coli Strain Derived from Oreochromis spp. Farms Using Whole-Genome Sequencing
09:44

Characterization of a Pathogenic Escherichia coli Strain Derived from Oreochromis spp. Farms Using Whole-Genome Sequencing

Published on: December 23, 2022

3.1K

Related Experiment Videos

Last Updated: Apr 1, 2026

Heuristic Mining of Hierarchical Genotypes and Accessory Genome Loci in Bacterial Populations
08:03

Heuristic Mining of Hierarchical Genotypes and Accessory Genome Loci in Bacterial Populations

Published on: December 7, 2021

2.9K
Generating Whole Bacterial Genomes from Clinical Samples using a Target Enrichment Workflow
10:44

Generating Whole Bacterial Genomes from Clinical Samples using a Target Enrichment Workflow

Published on: August 15, 2025

1.3K
Characterization of a Pathogenic Escherichia coli Strain Derived from Oreochromis spp. Farms Using Whole-Genome Sequencing
09:44

Characterization of a Pathogenic Escherichia coli Strain Derived from Oreochromis spp. Farms Using Whole-Genome Sequencing

Published on: December 23, 2022

3.1K

Area of Science:

  • Microbiology
  • Genomics
  • Epidemiology

Background:

  • Whole genome sequencing is a routine method in molecular epidemiology.
  • There is a need for a centralized database for bacterial whole genome sequencing data analysis.

Purpose of the Study:

  • To present BacWGSTdb, a bacterial whole genome sequence typing database.
  • To provide a platform for clinicians, microbiologists, and epidemiologists to analyze bacterial isolates.

Main Methods:

  • The database utilizes a hierarchical structure: species, clonal complex, and isolates.
  • It supports bacterial genotyping at both multi-locus sequence typing (MLST) and whole-genome levels.
  • Users can upload pre-assembled genome sequences for analysis.

Main Results:

  • BacWGSTdb identifies phylogenetically close isolates in the public database.
  • It provides associated clinical information, including host, isolation source, disease, collection time, and geographical location.
  • The database facilitates rapid comparison of query isolates to existing data.

Conclusions:

  • BacWGSTdb offers a rapid and convenient platform for bacterial genotyping.
  • It aids in addressing clinical microbiology issues, such as tracking bacterial pathogens.
  • The database supports molecular epidemiology and public health surveillance.